LOCUS       sequence-281216-        2636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0442
FEATURES             Location/Qualifiers
     source          1..2636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaga tgggcttgaa ttacaacaag agccaaactc attttttgat
       61 gcaacagaca gggtctcgtt ctgtcaccca ggctggagca cagtggcgcc atctgggtcc
      121 actgcaacct ccgcctccca ggctcaagtg atctgctcag cttcccgagt aactggggct
      181 acaggagctg atggtacaca catggatggt gatcaaattg ttgtggaagt acaagaaact
      241 gtttttgttt cagatgttgt ggattcagac ataactgtgc ataactttgt tcctgatgac
      301 ccagattcag ttgtaatcca agatgttatt gaggacgttg ttatagaaga tgttcagtgc
      361 ccagatatca tggaagaagc agatgtgtct gaaacggtca tcattcctga gcaagtgctg
      421 gactcagatg taactgaaga agtttcttta gcacattgca cagtcccaga tgatgtttta
      481 gcttctgaca ttacttcagc ctcaatgtct atgccagaac acgtcttgac gggtgattct
      541 atacatgtgt ctgacgttgg acatgttgga catgttggac atgttgaaca tgtggttcat
      601 gatagtgtag tggaagcaga aattgtcact gatcctctga ctaccgacgt agtttcagaa
      661 gaagtattgg tagcagactg tgcctctgaa gcagtcatag atgccaatgg gatccctgtg
      721 gaccagcagg atgatgacaa aggcaactgt gaggactacc ttatgatttc cttggatgat
      781 gctggcaaaa tagaacacga tggttcttct ggaatgacca tggacacaga gtcggaaatt
      841 gatccttgta aagtggatgg cacttgccct gaggtcatca aggtgtacat ttttaaagct
      901 gaccctggag aagatgactt aggtggaact gtagacattg tggagagtga gcctgagaat
      961 gatcatggag ttgaactgct tgatcagaac agcagtattc gtgttcccag ggaaaagatg
     1021 gtttatatga ctgtcaatga ctctcagcca gaagatgaag atttaaatgt tgctgaaatc
     1081 gctgacgaag tttatatgga agtgatcgta ggagaggagg atgctgcagc agcagcggca
     1141 gccgccgccg tgcacgagca gcaaatggat gacaatgaaa tcaaaacctt catgccgatt
     1201 gcatgggcag cagcttatgg taataattct gatggaattg aaaaccggaa tggcactgca
     1261 agtgccctct tgcacataga tgagtctgct ggcctcggca gactggctaa acaaaaacca
     1321 aagaaaagga gaagacctga ttccaggcag taccaaacag caataattat tggccctgat
     1381 ggacatcctt tgactgtcta tccttgcatg atttgtggga agaagtttaa gtcgagaggt
     1441 tttttgaaaa ggcacatgaa aaaccatccc gaacaccttg ccaagaagaa ataccgctgt
     1501 actgactgtg attacactac caacaagaag ataagtttac acaaccacct ggagagccac
     1561 aagctgacca gcaaggcaga gaaggccatt gaatgcgatg agtgtgggaa gcatttctct
     1621 catgcagggg ctttgtttac tcacaaaatg gtgcataagg aaaaaggagc caacaaaatg
     1681 cacaagtgta aattctgtga atacgagaca gctgaacaag ggttattgaa tcgccacctc
     1741 ttggcagtcc acagcaagaa ctttcctcat atttgtgtgg agtgtggtaa gggttttcgt
     1801 cacccgtcag agctcaaaaa gcacatgaga atccatactg gggagaagcc gtaccaatgc
     1861 cagtactgcg aatataggtc tgcagactct tctaacttga aaacgcatgt caaaactaag
     1921 catagtaaag agatgccatt caagtgtgac atttgtcttc tgactttctc ggataccaaa
     1981 gaggtgcagc aacatgctct tatccaccaa gaaagcaaaa cacaccagtg tttgcattgc
     2041 gaccacaaga gttcgaactc aagtgatttg aaacgacaca taatttcagt tcacacgaaa
     2101 gactaccccc ataagtgtga catgtgtgat aaaggctttc acaggccttc agaactcaag
     2161 aaacacgtgg ctgcccacaa gggcaaaaaa atgcaccagt gtagacattg tgactttaag
     2221 attgcagatc catttgttct aagtcgccat attctctcag ttcacacaaa ggatcttcca
     2281 tttaggtgca agagatgtag aaagggattt aggcaacaga gtgagcttaa aaagcatatg
     2341 aagacacaca gtggcaggaa agtgtatcag tgtgagtact gtgagtatag cactacagat
     2401 gcctcaggct ttaaacggca cgttatttcc attcacacga aagactatcc tcaccggtgt
     2461 gagtactgca agaaaggctt ccgaagacct tcagaaaaga accagcacat aatgcgacat
     2521 cataaagaag ttggcctgcc ctagtaatga actagtccgt aacttgaaag tatttcgatt
     2581 tcttggcttt atatatcttg tggaaaggac gatctaaaga ctatgatgtc tccttg
//
