LOCUS       sequence-281219-        5000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0449
FEATURES             Location/Qualifiers
     source          1..5000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgccggcgcg taccgcccca gcccgggtgc ccacactggc cgtcccggcc
       61 atctcgctgc ccgacgatgt ccgcaggcgg ctcaaagatt tggaaagaga cagcttaaca
      121 gaaaaggaat gtgtgaagga gaaattgaat ctcttgcacg aatttctgca aacagaaata
      181 aagaatcagt tatgtgactt ggaaaccaaa ttacgtaaag aagaattatc cgaggagggc
      241 tacctggcta aagtcaaatc ccttttaaat aaagatttgt ccttggagaa cggtgctcat
      301 gcttacaacc gggaagtgaa tggacgtcta gaaaacggga accaagcaag aagtgaagcc
      361 cgtagagtgg gaatggcaga tgccaacagc ccccccaaac ccctttccaa acctcgcacg
      421 cccaggagga gcaagtccga tggagaggct aagcgttcaa gagaccctcc tgcctcagcc
      481 tcccaagtaa ctgggattag agctgaacct tcacctagcc ccaggattac aaggaaaagc
      541 accaggcaaa ccaccatcac atctcatttt gcaaagggcc ctgccaaacg gaaacctcag
      601 gaagagtctg aaagagccaa atcggatgag tccatcaagg aagaagacaa agaccaggat
      661 gagaagagac gtagagttac atccagagaa cgagttgcta gaccgcttcc tgcagaagaa
      721 cctgaaagag caaaatcagg aacgcgcact gaaaaggaag aagaaagaga tgaaaaagaa
      781 gaaaagagac tccgaagtca aaccaaagaa ccaacaccca aacagaaact gaaggaggag
      841 ccggacagag aagccagggc aggcgtgcag gctgacgagg acgaagatgg agacgagaaa
      901 gatgagaaga agcacagaag tcaacccaaa gatctagctg ccaaacggag gcccgaagaa
      961 aaagaacctg aaaaagtaaa tccacagatt tctgatgaaa aagacgagga tgaaaaggag
     1021 gagaagagac gcaaaacgac ccccaaagaa ccaacggaga aaaaaatggc tcgcgccaaa
     1081 acagtcatga actccaagac ccaccctccc aagtgcattc agtgcgggca gtacctggac
     1141 gaccctgacc tcaaatatgg gcagcaccca ccagacgcgg tggatgagcc acagatgctg
     1201 acaaatgaga agctgtccat ctttgatgcc aacgagtctg gctttgagag ttatgaggcg
     1261 cttccccagc acaaactgac ctgcttcagt gtgtactgta agcacggtca cctgtgtccc
     1321 atcgacaccg gcctcatcga gaagaatatc gaactcttct tttctggttc agcaaaacca
     1381 atctatgatg atgacccatc tcttgaaggt ggtgttaatg gcaaaaatct tggccccata
     1441 aatgaatggt ggatcactgg ctttgatgga ggtgaaaagg ccctcatcgg cttcagcacc
     1501 tcatttgccg aatacattct gatggatccc agtcccgagt atgcgcccat atttgggctg
     1561 atgcaggaga agatctacat cagcaagatt gtggtggagt tcctgcagag caattccgac
     1621 tcgacctatg aggacctgat caacaagatc gagaccacgg ttcctccttc tggcctcaac
     1681 ttgaaccgct tcacagagga ctccctcctg cgacacgcgc agtttgtggt ggagcaggtg
     1741 gagagttatg acgaggccgg ggacagtgat gagcagccca tcttcctgac accctgcatg
     1801 cgggacctga tcaagctggc tggggtcacg ctgggacaga ggcgagccca ggcgaggcgg
     1861 cagaccatca ggcattctac cagggagaag gacaggggac ccacgaaagc caccaccacc
     1921 aagctggtct accagatctt cgatactttc ttcgcagagc aaattgaaaa ggatgacaga
     1981 gaagacaagg agaacgcctt taagcgccgg cgatgtggcg tctgtgaggt gtgtcagcag
     2041 cctgagtgtg ggaaatgtaa agcctgcaag gacatggtta aatttggtgg cagtggacgg
     2101 agcaagcagg cttgccaaga gcggaggtgt cccaatatgg ccatgaagga ggcagatgac
     2161 gatgaggaag tcgatgataa catcccagag atgccgtcac ccaaaaaaat gcaccagggg
     2221 aagaagaaga aacagaacaa gaatcgcatc tcttgggtcg gagaagccgt caagactgat
     2281 gggaagaaga gttactataa gaaggtgtgc attgatgcgg aaaccctgga agtgggggac
     2341 tgtgtctctg ttattccaga tgattcctca aaaccgctgt atctagcaag ggtcacggcg
     2401 ctgtgggagg acagcagcaa cgggcagatg tttcacgccc actggttctg cgctgggaca
     2461 gacacagtcc tcggggccac gtcggaccct ctggagctgt tcttggtgga tgaatgtgag
     2521 gacatgcagc tttcatatat ccacagcaaa gtgaaagtca tctacaaagc cccctccgaa
     2581 aactgggcca tggagggagg catggatccc gagtccctgc tggaggggga cgacgggaag
     2641 acctacttct accagctgtg gtatgatcaa gactacgcga gattcgagtc ccctccaaaa
     2701 acccagccaa cagaggacaa caagttcaaa ttctgtgtga gctgtgcccg tctggctgag
     2761 atgaggcaaa aagaaatccc cagggtcctg gagcagctcg aggacctgga tagccgggtc
     2821 ctctactact cagccaccaa gaacggcatc ctgtaccgag ttggtgatgg tgtgtacctg
     2881 ccccctgagg ccttcacgtt caacatcaag ctgtccagtc ccgtgaaacg cccacggaag
     2941 gagcccgtgg atgaggacct gtacccagag cactaccgga aatactccga ctacatcaaa
     3001 ggcagcaacc tggatgcccc tgagccctac cgaattggcc ggatcaaaga gatcttctgt
     3061 cccaagaaga gcaacggcag gcccaatgag actgacatca aaatccgggt caacaagttc
     3121 tacaggcctg agaacaccca caagtccact ccagcgagct accacgcaga catcaacctg
     3181 ctctactgga gcgacgagga ggccgtggtg gacttcaagg ctgtgcaggg ccgctgcacc
     3241 gtggagtatg gggaggacct gcccgagtgc gtccaggtgt actccatggg cggccccaac
     3301 cgcttctact tcctcgaggc ctataatgca aagagcaaaa gctttgaaga tcctcccaac
     3361 catgcccgta gccctggaaa caaagggaag ggcaagggaa aagggaaggg caagcccaag
     3421 tcccaagcct gtgagccgag cgagccagag atagagatca agctgcccaa gctgcggacc
     3481 ctggatgtgt tttctggctg cggggggttg tcggagggat tccaccaagc aggcatctct
     3541 gacacgctgt gggccatcga gatgtgggac cctgcggccc aggcgttccg gctgaacaac
     3601 cccggctcca cagtgttcac agaggactgc aacatcctgc tgaagctggt catggctggg
     3661 gagaccacca actcccgcgg ccagcggctg ccccagaagg gagacgtgga gatgctgtgc
     3721 ggcgggccgc cctgccaggg cttcagcggc atgaaccgct tcaattcgcg cacctactcc
     3781 aagttcaaaa actctctggt ggtttccttc ctcagctact gcgactacta ccggccccgg
     3841 ttcttcctcc tggagaatgt caggaacttt gtctccttca agcgctccat ggtcctgaag
     3901 ctcaccctcc gctgcctggt ccgcatgggc tatcagtgca ccttcggcgt gctgcaggcc
     3961 ggtcagtacg gcgtggccca gactaggagg cgggccatca tcctggccgc ggcccctgga
     4021 gagaagctcc ctctgttccc ggagccactg cacgtgtttg ctccccgggc ctgccagctg
     4081 agcgtggtgg tggatgacaa gaagtttgtg agcaacataa ccaggttgag ctcgggtcct
     4141 ttccggacca tcacggtgcg agacacgatg tccgacctgc cggaggtgcg gaatggagcc
     4201 tcggcactgg agatctccta caacggggag cctcagtcct ggttccagag gcagctccgg
     4261 ggcgcacagt accagcccat cctcagggac cacatctgta aggacatgag tgcattggtg
     4321 gctgcccgca tgcggcacat ccccttggcc ccagggtcag actggcgcga tctgcccaac
     4381 atcgaggtgc ggctctcaga cggcaccatg gccaggaagc tgcggtatac ccaccatgac
     4441 aggaagaacg gccgcagcag ctctggggcc ctccgtgggg tctgctcctg cgtggaagcc
     4501 ggcaaagcct gcgaccccgc agccaggcag ttcaacaccc tcatcccctg gtgcctgccc
     4561 cacaccggga accggcacaa ccactgggct ggcctctatg gaaggctcga gtgggacggc
     4621 ttcttcagca caaccgtcac caaccccgag cccatgggca agcagggccg cgtgctccac
     4681 ccagagcagc accgtgtggt gagcgtgcgg gagtgtgccc gctcccaggg cttccctgac
     4741 acctaccggc tcttcggcaa catcctggac aagcaccggc aggtgggcaa tgccgtgcca
     4801 ccgcccctgg ccaaagccat tggcttggag atcaagcttt gtatgttggc caaagcccga
     4861 gagagtgcct cagctaaaat aaaggaggag gaagctgcta aggactagta atgaactagt
     4921 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacct
     4981 atgcatggtg tgcttcattt
//
