LOCUS       sequence-281222-        2012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0487
FEATURES             Location/Qualifiers
     source          1..2012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccccgag gcctccgacc gccgcgcccc aggaatcagt gacattcaaa
       61 gatgtgtctg tggacttcac ccaggaagaa tggtaccatg tcgaccctgc tcagaggagc
      121 ttatacaggg atgtgatgct ggagaactat agccacctgg tttctcttgg atatcaagtt
      181 tccaagccag aggtgatctt caaattggag caaggagaag agccatggat atcagaggga
      241 gaaatccaac gacctttcta tccagactgg aagaccaggc ctgaagtcaa atcatcacat
      301 ttgcagcagg atgtatcaga agtatcccac tgcacacatg atctcttaca tgctacatta
      361 gaagactcct gggatgttag cagccagtta gacaggcaac aggaaaactg gaagagacat
      421 ctgggatcag aggcatccac ccagaagaaa ataattacac cacaagaaaa ttttgagcaa
      481 aataaatttg gtgaaaattc tagattgaac accaatttgg ttacacaact gaacattcct
      541 gcaagaataa ggcctagtga atgtgagacc cttggaagca atttgggaca taatgcagac
      601 ttacttaatg agaataatat tcttgcaaaa aagaaaccct ataagtgtga taaatgtaga
      661 aaagccttta ttcatagatc atcgcttact aaacatgaga aaacacataa aggagaggga
      721 gctttcccta atggaacaga tcaaggaatt tatcctggaa agaaacacca tgaatgtacc
      781 gactgtggga aaacctttct ctggaagaca cagcttactg agcatcagag aattcacact
      841 ggggagaaac cctttgaatg caatgtatgt ggaaaggcct tcaggcatag ctcatctctt
      901 ggtcagcatg agaatgctca taccggagag aaaccctatc agtgtagtct ctgtgggaaa
      961 gccttccagc gcagctcctc ccttgttcaa caccagcgaa ttcacactgg agagaaaccc
     1021 tatcgatgta atctatgtgg gaggtccttt aggcatggca catccctcac tcaacacgag
     1081 gtcacacaca gtggagagaa gcccttccag tgtaaggaat gtgggaaagc ctttagtcga
     1141 tgttcttccc ttgtccaaca tgagaggact catactggag agaaaccttt tgaatgtagc
     1201 atatgtggga gggcttttgg tcagagccca tccctttata aacatatgag gattcataag
     1261 agaggcaaac cttaccaaag cagtaactac agcatagatt tcaagcacag cacatctctc
     1321 actcaagatg aaagcactct taccgaagtg aaatcctacc attgtaatga ctgtggggaa
     1381 gactttagtc acattacaga ctttactgac catcagagga tccatactgc agagaacccc
     1441 tatgattgtg agcaggcttt tagtcagcaa gctatttctc atcctggaga gaaaccctat
     1501 caatgtaatg tatgtgggaa agctttcaaa aggagtacaa gtttcataga gcatcacaga
     1561 attcatactg gagagaaacc ctatgaatgt aatgagtgtg gagaagcctt tagtcgacgc
     1621 tcatcgctta ctcaacatga gagaacccac actggagaga aaccctatga atgtattgac
     1681 tgtgggaaag cctttagtca aagttcatct ctcattcagc atgagagaac tcatactgga
     1741 gagaagccct atgaatgtaa tgaatgtggg agagccttcc gaaaaaaaac caacctgcat
     1801 gatcatcaga gaattcatac tggagaaaaa ccctattctt gtaaggaatg tgggaaaaac
     1861 ttcagccgaa gttcagctct tactaaacac cagagaattc atactcgaaa taaactctag
     1921 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1981 aaggacgagt agcccttacg tgcacaatta tg
//
