LOCUS       sequence-281228-        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0490
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctttaga tctaagaacc atatttcagt gtgaaccatc cgagaataat
       61 cttggatcag aaaattcagc gtttcaacaa agccaaggac ctgctgttca gagagaagaa
      121 gggatttctg agttctcaag aatggtgctc aattcatttc aagacagcaa taattcatat
      181 gcaaggcagg aattgcaaag actttatagg atctttcact catggctgca accagaaaag
      241 cacagcaagg atgaaattat ttctctatta gtcctggagc agtttatgat tggtggccac
      301 tgcaatgaca aagccagtgt gaaagagaaa tggaaatcaa gtggcaaaaa cttggagaga
      361 ttcatagaag acctgactga tgacagcata aatccacctg ccttagtcca cgtccacatg
      421 cagggacagg aagctctctt ttctgaggat atgcccttaa gagatgtcat tgttcatctc
      481 acaaaacaag tgaatgccca aaccacaaga gaagcaaaca tggggacacc ctcccagact
      541 tcccaagata cttccttaga aacaggacaa ggatatgaag atgaacaaga tggctggaac
      601 agttcttcga aaactactcg agtaaatgaa aatattacta atcaaggcaa tcaaatagtt
      661 tccctaatca tcatccagga agagaacggt cctaggcctg aagagggagg tgtttcttct
      721 gacaacccat acaactcaaa aagagcagag ctagtcactg ctagatctca ggaagggtcc
      781 ataaatggaa tcactttcca aggtgtccct atggtgatgg gagcagggtg tatctctcaa
      841 ccagagcagt cctcccctga gtctgccctt acccaccaga gcaatgaggg aaattccaca
      901 tgtgaggtac atcagaaagg atcccatgga gtccaaaaat catacaaatg tgaagaatgc
      961 cccaaggtct ttaagtatct ctgtcactta ttagctcacc agagaagaca caggaatgag
     1021 aggccatttg tttgtcccga gtgtcaaaaa ggcttcttcc agatatcaga cctacgggtg
     1081 catcagataa ttcacacagg aaagaagcct ttcacatgca gcatgtgtaa aaagtccttc
     1141 agccacaaaa ccaacctgcg gtctcatgag agaatccaca caggagaaaa gccttataca
     1201 tgtccctttt gtaagacaag ctaccgccag tcatccacat accaccgcca tatgaggact
     1261 catgagaaaa ttaccctgcc aagtgttccc tccacaccag aagcttccta gtaatgaact
     1321 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1381 gatatgtgta cagatcgatt gtc
//
