LOCUS       sequence-281234-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0503
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgagga cctctctgcg gccacgtcct acaccgaaga tgatttctac
       61 tgccccgtct gtcaggaggt gctcaaaacg cccgtgcgga ccacggcctg tcagcacgtc
      121 aataggagtg aaacatccac atctgataac acagaaactt accaagagaa tacaagttct
      181 tctggtcatc ctacttttaa gtgtcccctg tgtcaagaat caaattttac cagacagcgt
      241 ttactggatc actgtaacag taatcaccta tttcagatag ttcctgtgac atgtcctatt
      301 tgtgtgtctc ttccttgggg agatcctagc cagattacca gaaatttcgt tagtcatcta
      361 aatcagagac atcaatttga ttatggagaa tttgtgaatc ttcagctaga tgaagaaacc
      421 caataccaaa ctgctgttga agaatctttt caagtaaaca tctagtaatg aactagtccg
      481 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatccgaac
      541 ctgtagaacc gaaggtt
//
