LOCUS       sequence-281237-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0505
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaactt ccaaaaggtg gaaaagatcg gagagggcac gtacggagtt
       61 gtgtacaaag ccagaaacaa gttgacggga gaggtggtgg cgcttaagaa aatccgcctg
      121 gacacgctgc tggatgtcat tcacacagaa aataaactct acctggtttt tgaatttctg
      181 caccaagatc tcaagaaatt catggatgcc tctgctctca ctggcattcc tcttcccctc
      241 atcaagagct atctgttcca gctgctccag ggcctagctt tctgccattc tcatcgggtc
      301 ctccaccgag accttaaacc tcagaatctg cttattaaca cagagggggc catcaagcta
      361 gcagactttg gactagccag agcttttgga gtccctgttc gtacttacac ccatgaggtg
      421 actcgccggg ccctattccc tggagattct gagattgacc agctcttccg gatctttcgg
      481 actctgggga ccccagatga ggtggtgtgg ccaggagtta cttctatgcc tgattacaag
      541 ccaagtttcc ccaagtgggc ccggcaagat tttagtaaag ttgtacctcc cctggatgaa
      601 gatggacgga gcttgttatc gcaaatgctg cactacgacc ctaacaagcg gatttcggcc
      661 aaggcagccc tggctcaccc tttcttccag gatgtgacca agccagtacc ccatcttcga
      721 ctctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      781 tgtggaaagg acgattactc tggtggtgaa agacgcat
//
