LOCUS       sequence-28125-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peCALWY-NB
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(572..593)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aggagttaag tatgacattg acctgcccaa caagaaggtc tgcattgaat ctgagcacag
       61 catggacact ctgcttgcaa ccctgaagaa aacaggaaag actgtttcct accttggcct
      121 tgagctcatt cgtggcggat ccggcggaag cggcggatcc ggcggtagcg gcggatccgg
      181 cggctccggc ggatccggcg gcagcggcgg atccggtgga agcggtggat ccggtggtag
      241 cggtggatcc ggtggaagcg gtggatccgg tggtagcggt ggatccgggg gtccgcggat
      301 gcagggcaca tgcagtacca ctctgattgc cattgccggc atgaccagtg catccagtgt
      361 ccattccatt gaaggcatga tctcccaact ggaaggggtg cagcaaatat cggtgtcttt
      421 ggccgaaggg actgcaacag ttctttataa tcccgctgta attagcccag aagaactcag
      481 agctgctata gaagacatgg gatttgaggc ttcagtctcg gtacccatgg tgagcaaggg
      541 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      601 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacgg
//
