LOCUS       sequence-281252-        1664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0527
FEATURES             Location/Qualifiers
     source          1..1664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccct tcccggcacg gtaccgagaa tgatgcggcc ggctccgggg
       61 cagaactacc cccgcacggg attccctttg gaagtgtcca ccccgcttgg ccaaggccgg
      121 gtcaatcagc tgggaggggt cttcatcaat gggcgacccc tgcctaacca catccgccac
      181 aagatagtgg agatggccca ccatggcatc cggccctgtg tcatctcccg acagctgcgt
      241 gtctcccacg gctgcgtctc caagattctt tgccgctacc aggagaccgg gtccatccgg
      301 cctggggcca tcggcggcag caagcccaga caggtggcga ctccggatgt agagaaaaag
      361 attgaggagt acaagaggga aaacccaggc atgttcagct gggagatccg ggacaggctg
      421 ctgaaggatg ggcactgtga ccgaagcact gtgccctcag gtttagtgag ttcgattagc
      481 cgcgtgctca gaatcaagtt cgggaagaaa gaggaggagg atgaagcgga caagaaggag
      541 gacgacggcg aaaagaaggc caaacacagc atcgacggca tcctgggcga caaagggaac
      601 cggctggacg agggctcgga tgtggagtcg gaacctgacc tcccactgaa gcgcaagcag
      661 cgacgcagtc ggaccacatt cacggccgag cagctggagg agctggagaa ggcctttgag
      721 aggacccact acccagacat atacacccgc gaggagctgg cgcagaggac caagctgaca
      781 gaggcgcgtg tgcaggtctg gttcagtaac cgccgcgccc gttggcgtaa gcaggcagga
      841 gccaaccagc tggcggcgtt caaccacctt ctgccaggag gcttcccacc caccggcatg
      901 cccacgctgc ccccctacca gctgccggac tccacctacc ccaccaccac catctcccaa
      961 gatgggggca gcactgtgca ccggcctcag cccctgccac cgtccaccat gcaccagggc
     1021 gggctggctg cagcggctgc agccgccgac accagctctg cctacggagc ccgccacagc
     1081 ttctccagct actctgacag cttcatgaat ccggcggcgc cctccaacca catgaacccg
     1141 gtcagcaacg gcctgtctcc tcaggtgatg agcatcttgg gcaaccccag tgcggtgccc
     1201 ccgcagccac aggctgactt ctccatctcc ccgctgcatg gcggcctgga ctcggccacc
     1261 tccatctcag ccagctgcag ccagcgggcc gactccatca agccaggaga cagcctgccc
     1321 acctcccagg cctactgccc acccacctac agcaccaccg gctacagcgt ggaccccgtg
     1381 gccggctatc agtacggcca gtacggccag agtgagtgcc tggtgccctg ggcgtccccc
     1441 gtccccattc cttctcccac ccccagggcc tcctgcttgt ttatggagag ctacaaggtg
     1501 gtgtcagggt ggggaatgtc catttcacag atggaaaaat tgaagtccag ccagatggaa
     1561 cagttcacct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1621 atatcttgtg gaaaggacga ctgtttcctc aatgtattgc aaca
//
