LOCUS       sequence-281258-        3647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0546
FEATURES             Location/Qualifiers
     source          1..3647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagacgcg ggcggcagaa aacacggcca tctttatgtg taaatgttgt
       61 aacctcttct caccaaatca gtcggaactc ctctcccacg tttcagagaa gcacatggaa
      121 gaaggggtta atgttgatga gattattatt ccccttaggc ctctgagtac acctgaaccc
      181 cccaactcaa gcaaaaccgg agatgagttt ttggtcatga agaggaagag aggcaggcct
      241 aaggggtcca cgaagaagtc cagcacagaa gaggagctgg cagaaaacat cgtgagtccg
      301 actgaggaca gcccgctggc tccggaggaa gggaacagcc tgcctccaag cagcttggag
      361 tgtagcaagt gctgtcggaa gttctccaac acgcgccagc tgcggaagca catctgcatt
      421 atcgtgctga atttgggtga ggaggaagga gaagcaggtg ctaccaagat tgtgccagtg
      481 gaggctgggc cccctgaaac aggagctaca aattctgaga ccacttcagc agacctggtg
      541 cctcggagag gctaccagga atacgccatt cagcagacac cttatgagca accaatgaag
      601 tcaagcaggc taggtcccac tcagctcaaa atcttcactt gtgaatactg caacaaggtc
      661 ttcaagttca agcactcgct gcaggcccac ctgaggatcc acaccaatga aaagccatac
      721 aagtgccccc agtgcagcta tgccagtgcc atcaaggcca acctcaatgt gcacctgcgc
      781 aagcacactg gagagaagtt cgcctgcgac tattgctcgt tcacctgcct gagcaagggc
      841 cacctcaagg tgcacatcga gcgagtgcac aagaagatca agcagcactg ccgcttctgc
      901 aagaagaagt actctgacgt caagaacctc atcaagcaca tccgagacgc gcatgaccca
      961 caggacaaga aggtcaaaga ggccttggac gagctctgcc tgatgacgag ggagggcaag
     1021 cggcagctgc tctatgactg ccacatctgt gagcgcaagt tcaagaacga gctggaccgt
     1081 gaccgccata tgctggtcca cggagacaag tggccttttg cctgtgagct ctgtggccat
     1141 ggggccacca agtaccaggc gctggaactg catgtcagga agcacccctt cgtgtacgtc
     1201 tgtgccgtct gccgcaagaa gttcgtcagc tccatcaggc tgcgcaccca catcaaagag
     1261 gtgcacgggg ctgcccagga ggccttggtc ttcaccagtt ccatcaacca gagcttctgc
     1321 ctcctggaac ctggtgggga catccagcaa gaagctctgg gggaccagct acagctggtg
     1381 gaagaggagt ttgccctcca gggcgtgaat gcactcaagg aagaggcctg tcctggggac
     1441 actcagctgg aggagggccg gaaggagccg gaggcccctg gggaaatgcc tgccccagct
     1501 gtgcacctgg cctccccgca ggccgaaagc acagccctgc caccctgtga gctggaaacc
     1561 accgtggtct cctcctcaga cctgcattct caagaggtgg tttcagatga ttttttgttg
     1621 aaaaatgata cctcctccgc agaggctcat gctgctcctg agaagccccc agacatgcag
     1681 cacagaagct cagtccagac gcaaggtgaa gtgatcacac tactgctgtc caaggcccag
     1741 agtgctgggt cagatcagga aagccatggc gcccagagcc ccctagggga agggcagaac
     1801 atggctgtgc tttcagctgg tgacccagat cccagcaggt gtctcaggtc aaacccagct
     1861 gaggcctcag acctcctccc tccagtagct ggtggtgggg acaccatcac acatcagcct
     1921 gactcttgca aagctgcccc tgagcaccgg tcaggcatca ccgctttcat gaaggtcctg
     1981 aacagtttac agaagaagca aatgaacacc agcttgtgtg agcggatccg gaaggtttat
     2041 ggagacctgg agtgtgaata ctgtggcaaa cttttttggt accaagtgca ttttgatatg
     2101 catgtccgca cccacacccg ggaacatctg tattattgct ctcagtgtca ttattcttcc
     2161 atcaccaaaa actgccttaa acgccacgta attcagaaac acagtaacat cttgctgaag
     2221 tgtcccaccg atggctgtga ctactcaact ccagataaat ataagctaca ggcacatctt
     2281 aaagttcaca cagcactgga caaaaggagt tattcttgtc ctgtttgtga aaagtctttt
     2341 tcagaggatc gattgataaa gtcacatatc aagaccaacc atcctgaggt ctccatgagc
     2401 accatttctg aggttctcgg gaggagggtt cagctgaaag ggctaattgg aaagagagcc
     2461 atgaaatgcc catattgtga cttttatttc atgaagaatg gctcagacct tcagcgtcat
     2521 atttgggctc atgaaggtgt gaagcccttc aagtgttctt tgtgtgagta tgcaactcgt
     2581 agcaagagta acctcaaggc tcatatgaat cgtcacagca ctgagaaaac ccacctatgt
     2641 gacatgtgtg gcaagaaatt caaatcaaaa gggacactga aaagtcacaa actccttcac
     2701 actgcagatg ggaagcagtt taagtgcacg gtgtgtgact acacagcggc ccagaagcca
     2761 cagctgctgc ggcacatgga acagcatgtc tccttcaagc ctttccgctg tgcccattgc
     2821 cattactcct gcaacatatc tggctctctg aagcggcact acaacaggaa gcaccctaat
     2881 gaggagtatg ccaacgtggg caccggggag ctggcagcgg aggtgctcat ccagcaaggt
     2941 ggtttgaagt gtcctgtttg cagctttgta tatggcacca aatgggagtt caataggcac
     3001 ttgaagaaca aacatggctt gaaggtggtg gaaattgatg gagaccccaa gtgggagaca
     3061 gcaacagaag ctcctgagga gccctccacc cagtatctcc acatcacaga ggccgaagaa
     3121 gacgttcaag ggacacaggc agcggtggcc gcgctccagg acctgagata cacctctgag
     3181 agtggcgacc gactggaccc cacggccgtg aacatcctgc agcagatcat tgagctgggc
     3241 gccgagaccc atgacgccac tgcccttgcc tcggtggttg ccatggcacc agggacggtg
     3301 actgtggtta agcaggtcac cgaggaggag cccagctcca accacacggt catgatccag
     3361 gagacggtcc agcaagcgtc cgtggagctt gccgagcagc accacctggt ggtgtcctcc
     3421 gacgacgtgg agggcattga gacggtgact gtctacacgc agggcgggga ggcctcggag
     3481 ttcatcgtct acgtgcagga ggccatgcag cctgtggagg agcaggctgt ggagcagccg
     3541 gcccaggaac tctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3601 tatatatctt gtggaaagga cgatgccacg tcccgcgctc atatatt
//
