LOCUS       sequence-281270-        1805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0564
FEATURES             Location/Qualifiers
     source          1..1805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggccagcc tgcaccctac gcagagggcc ctatacaggg aggtgatgct
       61 ggagaattat gcaaatgtga cttccttgtc agcatttcca ttccccaaac cagatctgat
      121 attccagctg gagcaaggag aagcagcatg gggcccagat ccctggacac ttgctggggg
      181 agaggccctg agaggcatgt gcacagagtt tcacttttgt tgcccaggct ggagtgcaat
      241 ggcgtgatct tcactcaccg caacctctgc cttccaggtg gtaaaaccaa gactgagaat
      301 gaggaaaaaa ctgcacagct aaacatttct aaagaatcag agtcccacag actgatagtg
      361 gagggactgc tgatggacgt tccccagcac cctgacttca aggacaggtt agagaagtca
      421 cagctacatg atacagggaa taaaacaaag ataggggatt gcacagattt gacagtccag
      481 gatcatgaat cttccaccac tgaaagggag gagatagcca ggaaattgga agaaagtagt
      541 gtcagcacac atctcattac aaagcagggt tttgccaaag aacaggtgtt ttataaatgt
      601 ggtgagtgtg gcagttacta caacccacat tcagactttc acctgcatca gagagttcac
      661 actaatgaga agccctacac atgcaaagaa tgtgggaaaa ccttcagata taactcaaaa
      721 ctgtcacggc atcagaaaat ccacactgga gagaaaccat actcatgtga ggaatgtgga
      781 caagccttca gtcaaaattc ccaccttctt cagcatcaga agctccatgg tggacagagg
      841 ccctatgaat gtactgactg tggtaaaacc ttcagttata attcaaaact gattcggcat
      901 cagagaatcc atactgggga gaagcccttt aaatgtaagg aatgtgggaa agccttcagt
      961 tgtagctatg actgcatcat ccatgaacga attcacaatg gggagaagcc ctatgaatgt
     1021 aaggagtgtg ggaagagttt gagttctaat tcagttctga ttcagcatca gagaatccac
     1081 actggggaga aaccttatga atgtaaagag tgtggcaagg ccttccaccg cagttcggta
     1141 tttcttcagc accagaggtt ccacactggg gaacaactct acaaatgtaa tgaatgttgg
     1201 aaaactttca gctgtagctc ccgcttcata gtgcatcaga gaatccacaa tggggagaaa
     1261 ccctatgaat gccaggaatg tgggaagaca ttcagtcaaa agatcactct ggttcagcat
     1321 cagcgagttc acactgggga gaaaccttat gagtgtaagg agtgcgggaa agccttcaga
     1381 tggaatgcaa gtttcatcca gcatcagaag tggcatacta ggaagaaact catcaatgga
     1441 acagggctat ccgcagttaa gccctactgt ccctgcgcca tcctctctcc tctgcctccc
     1501 caacatacct gctctgccct agccccacca gggcctccct tatcttcttc acatgcagtg
     1561 gtacttcctc cctctgtgcc tttcttcctg ctgcttccct catctgaaaa ggccaaccct
     1621 tcacctgtcc aaatagcaca tttttttcag gatctcgctt ttcctgggaa gtcatccctt
     1681 cagagcccga atcctttgtc tcactccctg tagtaatgaa ctagtccgta acttgaaagt
     1741 atttcgattt cttggcttta tatatcttgt ggaaaggacg acgatggaga tgacggccca
     1801 acggc
//
