LOCUS       sequence-281279-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0573
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             211..240
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             214..243
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             217..246
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             220..249
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tggaacaccc gctctttggc tgcctgcgca gccctcacgc cacggcgcaa
       61 ggcttgcacc cgttctccca atcctctctc gccctccatg gaagatctga ccatatgtct
      121 taccccgagc tctctacttc ttcctcatct tgcataatcg cgggataccc caacgaagag
      181 ggcatgtttg ccagccagca tcacaggggg caccaccacc accaccacca ccaccaccat
      241 caccaccatc agcagcagca gcaccaggct ctgcaaacca actggcacct cccgcagatg
      301 tcttccccac cgagtgcggc tcggcacagc ctctgcctcc agcccgactc tggagggccc
      361 ccagagttgg ggagcagccc gcccgtcctg tgctccaact cttccagctt gggctccagc
      421 accccgactg gggccgcgtg cgcgccgggg gactacggcc gccaggcact gtcacctgcg
      481 gaggcggaga agcgaagcgg cggcaagagg aaaagcgaca gctcagactc ccaggaagga
      541 aattacaagt cagaagtcaa cagcaaaccc aggaaagaaa ggacagcatt taccaaagag
      601 caaatcagag aacttgaagc agaatttgcc catcataatt atctcaccag actgaggcga
      661 tacgagatag cagtgaatct ggatctcact gaaagacagg tgaaagtctg gttccaaaac
      721 aggcggatga agtggaagag ggtaaagggt ggacagcaag gagctgcggc tcgggaaaag
      781 gaactggtga atgtgaaaaa gggaacactt ctcccatcag agctgtcggg aattggtgca
      841 gccaccctcc agcaaacagg ggactctata gcaaatgaag acagtcacga cagtgaccac
      901 agctcagagc atgcgcactt atagtaatga actagtccgt aacttgaaag tatttcgatt
      961 tcttggcttt atatatcttg tggaaaggac gaatgcgggc tcagggaaac ataagg
//
