LOCUS       sequence-281294-        1604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0595
FEATURES             Location/Qualifiers
     source          1..1604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccactgt cattcctggt gatttgtcag aagtaagaga tacccagaaa
       61 gtcccttcag ggaaacgtaa gcgtggtgaa accaaaccaa gaaaaaactt tccttgccaa
      121 ctgtgtgaca aggcctttaa cagtgttgag aaattaaagg ttcactccta ctctcacaca
      181 ggagagaggc cctacaagtg catacaacaa gactgcacca aggcctttgt ttctaagtac
      241 aaattacaaa ggcacatggc tactcattct cctgagaaaa cccacaagtg taattattgt
      301 gagaaaatgt ttcaccggaa agatcatctg aagaatcacc tccatacaca cgaccctaac
      361 aaagagacgt ttaagtgcga agaatgtggc aagaactaca ataccaagct tggatttaaa
      421 cgtcacttgg ccttgcatgc cgcaacaagt ggtgacctca cctgtaaggt atgtttgcaa
      481 acttttgaaa gcacgggagt gcttctggag caccttaaat ctcatgcagg caagtcgtct
      541 ggtggggtta aagaaaaaaa gcaccagtgc gaacattgtg atcgccggtt ctacacccga
      601 aaggatgtcc ggagacacat ggtggtgcac actggaagaa aggacttcct ctgtcagtat
      661 tgtgcacaga gatttgggcg aaaggatcac ctgactcgac atatgaagaa gagtcacaat
      721 caagagcttc tgaaggtcaa aacagaacca gtggatttcc ttgacccatt tacctgcaat
      781 gtgtctgtgc ctataaaaga cgagctcctt ccggtgatgt ccttaccttc cagtgaactg
      841 ttatcaaagc cattcacaaa cactttgcag ttaaacctct acaacactcc atttcagtcc
      901 atgcagagct cgggatctgc ccaccaaatg atcacaactt tacctttggg aatgacatgc
      961 ccaatagata tggacactgt tcatccctct caccaccttt ctttcaaata tccgttcagt
     1021 tctacctcat atgcaatttc tattcctgaa aaagaacagc cattaaaggg ggaaattgag
     1081 agttacctga tggagttaca aggtggcgtg ccctcttcat cccaagattc tcaagcatcg
     1141 tcatcatcta agctagggtt ggatcctcag attgggtccc tagatgatgg tgcaggagac
     1201 ctctccctat ccaaaagctc tatctccatc agtgaccccc taaacacacc agcattggat
     1261 ttttctcagt tgtttaattt cataccttta aatggtcctc cctataatcc tctatcagtg
     1321 gggagccttg gaatgagcta ttcccaggaa gaagcacatt cttctgtttc ccagctcccc
     1381 ccacaaacac aggatcttca ggatcctgca aacactatag ggcttgggtc tctgcactca
     1441 ctgtcagcag ctttcaccag cagtttaagc acaagtacca ccctcccacg tttccatcaa
     1501 gcttttcagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1561 atatcttgtg gaaaggacga ctaacgggtg ataacgcacg acca
//
