LOCUS       sequence-281297-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0605
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacggc gaccatagct ctcggcacag actccatcaa gatggagaac
       61 gggcagagca cagccgccaa gctggggctg cctcccctga cgcccgagca gcaggaggcc
      121 cttcagaagg ccaagaagta cgccatggag cagagcatca agagtgtgct ggtgaagcag
      181 accatcgcgc accagcagca gcagctcacc aacctgcaga tggcggctca gcggcagcgg
      241 gcgctggcca tcatgtgccg cgtctacgtg ggctctatct actatgagct gggggaggac
      301 accatccgcc aggcctttgc cccctttggc cccatcaaga gcatcgacat gtcctgggac
      361 tccgtcacca tgaagcacaa gggctttgcc ttcgtggagt atgaggtccc cgaagctgca
      421 cagctggcct tggagcagat gaactcggtg atgctggggg gcaggaacat caaggtgggc
      481 agacccagca acatagggca ggcccagccc atcatagacc agttggctga ggaggcacgg
      541 gccttcaacc gcatctacgt ggcctctgtg caccaggacc tctcagacga tgacatcaag
      601 agcgtgtttg aggcctttgg caagatcaag tcctgcacac tggcccggga ccccacaact
      661 ggcaagcaca agggctacgg cttcattgag tacgagaagg cccagtcgtc ccaagatgct
      721 gtgtcttcca tgaacctctt tgacctgggt ggccagtact tgcgggtggg caaggctgtc
      781 acaccgccca tgcccctact cacaccagcc acgcctggag gcctcccacc tgccgctgct
      841 gtggcagctg ctgcagccac tgccaagatc acagctcagg aagcagtggc cggagcagcg
      901 gtgctgggta ccctgggcac acctggactg gtgtccccag cactgaccct ggcccagccc
      961 ctgggcactt tgccccaggc tgtcatggct gcccaggcac ctggagtcat cacaggtgtg
     1021 accccagccc gtcctcctat cccggtcacc atcccctcgg tgggagtggt gaaccccatc
     1081 ctggccagcc ctccaacgct gggtctcctg gagcccaaga aggagaagga agaagaggag
     1141 ctgtttcccg agtcagagcg gccagagatg ctgagcgagc aggagcacat gagcatctcg
     1201 ggcagtagcg cccgacacat ggtgatgcag aagctgctcc gcaagcagga gtctacagtg
     1261 atggttctgc gcaacatggt ggaccccaag gacatcgatg atgacctgga aggggaggtg
     1321 acagaggagt gtggcaagtt cggggccgtg aaccgcgtca tcatctacca agagaaacaa
     1381 ggcgaggagg aggatgcaga aatcattgtc aagatctttg tggagttttc catagcctct
     1441 gagactcata aggccatcca ggccctcaat ggccgctggt ttgctggccg caaggtggtg
     1501 gctgaagtgt acgaccagga gcgttttgat aacagtgacc tctctgcgta gtaatgaact
     1561 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1621 cgacttaggc gggttatcca atg
//
