LOCUS       sequence-281300-        1565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0612
FEATURES             Location/Qualifiers
     source          1..1565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcctgt tgtgacaggg aaatttggtg agcggcctcc acctaaacga
       61 cttactaggg aagctatgcg aaattattta aaagagcgag gggatcaaac agtacttatt
      121 cttcatgcaa aagttgcaca gaagtcatat ggaaatgaaa aaaggttttt ttgcccacct
      181 ccttgtgtat atcttatggg cagtggatgg aagaaaaaaa aagaacaaat ggaacgcgat
      241 ggttgttctg aacaagagtc tcaaccgtgt gcatttattg ggataggaaa tagtgaccaa
      301 gaaatgcagc agctaaactt ggaaggaaag aactattgca cagccaaaac attgtatata
      361 tctgactcag acaagcgaaa gcacttcatg ttgtctgtaa agatgttcta tggcaacagt
      421 gatgacattg gtgtgttcct cagcaagcgg ataaaagtca tctccaaacc ttccaaaaag
      481 aagcagtcat tgaaaaatgc tgacttatgc attgcctcag gaacaaaggt ggctctgttt
      541 aatcgactac gatcccagac agttagtacc agatacttgc atgtagaagg aggtaatttt
      601 catgccagtt cacagcagtg gggagccttt tttattcatc tcttggatga tgatgaatca
      661 gaaggagaag aattcacagt ccgagatggc tacatccatt atggacaaac agtcaaactt
      721 gtgtgctcag ttactggcat ggcactccca agattgataa ttaggaaagt tgataagcag
      781 accgcattat tggatgcaga tgatcctgtg tcacaactcc ataaatgtgc attttacctt
      841 aaggatacag aaagaatgta tttgtgcctt tctcaagaaa gaataattca atttcaggcc
      901 actccatgtc caaaagaacc aaataaagag atgataaatg atggcgcttc ctggacaatc
      961 attagcacag ataaggcaga gtatacattt tatgagggaa tgggccctgt ccttgcccca
     1021 gtcactcctg tgcctgtggt agagagcctt cagttgaatg gcggtgggga cgtagcaatg
     1081 cttgaactta caggacagaa tttcactcca aatttacgag tgtggtttgg ggatgtagaa
     1141 gctgaaacta tgtacaggtg tggagagagt atgctctgtg tcgtcccaga catttctgca
     1201 ttccgagaag gttggagatg ggtccggcaa ccagtccagg ttccagtaac tttggtccga
     1261 aatgatggaa tcatttattc caccagcctt acctttacct acacaccaga accagggccg
     1321 cggccacatt gcagtgcagc aggagcaatc cttcgagcca attcaagcca ggtgccccct
     1381 aacgaatcaa acacaaacag cgagggaagt tacacaaacg ccagcacaaa ttcaaccagt
     1441 gtcacatcat ctacagccac agtggtatcc tagtaatgaa ctagtccgta acttgaaagt
     1501 atttcgattt cttggcttta tatatcttgt ggaaaggacg aagttatcgc cgtgatctcg
     1561 ggata
//
