LOCUS       sequence-281306-        1577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0621
FEATURES             Location/Qualifiers
     source          1..1577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccatccc caagcactcc ctgagcccag tgccgtggga agaggacagc
       61 ttccttcaag tgaaggtgga ggaggaagag gaagccagcc tctcccaggg cggagaatcc
      121 agccatgacc acattgctca ctctgaggct gcacgcctgc gcttccggca cttccgctat
      181 gaggaggcat ctggtccaca cgaggccctg gcccacctcc gagcgctgtg ctgtcagtgg
      241 ctgcagcccg aggcgcactc caaggagcag atactggagc tgctggtgct ggagcagttc
      301 ctgggtgcgc tgcccccaga gatccaagcc tgggtgggag cccagagtcc caagagcgga
      361 gaggaagccg ctgtgctggt ggaggatctg actcaggtgc tggacaagag aggatgggat
      421 ccaggagccg agcccacaga ggcaagctgc aagcagagtg acctgggaga gtcagagcca
      481 tcaaatgtca ctgagaccct catgggaggt gtttcccttg gacccgcctt tgtcaaggca
      541 tgtgaacctg agggcagctc agagaggtct ggactatcag gggagatctg gacaaagtct
      601 gtcacccaac agatccactt caagaaaact tcagggcctt acaaggatgt ccccacagac
      661 cagcgtggcc gtgaatctgg tgcctcgagg aacagttcta gtgcgtggcc aaacctcacc
      721 tcccaagaga agcctccttc agaagacaaa tttgatctgg tggatgctta tgggacagag
      781 cctccataca cctactcagg gaagaggtcc tccaagtgtc gcgagtgtag gaagatgttc
      841 cagagtgctt cggcgctcga ggcacaccag aagacccatt ctcggaagac cccatatgcc
      901 tgcagcgagt gtgggaaagc cttcagccgg agcactcacc tcgcccagca ccaggttgtc
      961 cacacagggg cgaagcccca tgagtgtaag gaatgtggga aggccttcag ccgagtcacc
     1021 cacctgactc agcaccaaag gattcatact ggagagaaac cctacaaatg tggggaatgt
     1081 ggtaaaacct tcagccgcag cactcacctc acccagcacc agcgggtgca cacgggggag
     1141 cggccctacg agtgtgacgc gtgtgggaaa gccttcagcc agagcacgca cctgactcaa
     1201 caccagcgca tccacaccgg ggagaagccc tacaagtgtg acgcgtgtgg ccgagccttc
     1261 agcgactgct cagccctgat ccgacatctg agaatccact ctggagagaa gccatatcag
     1321 tgtaaggttt gtccgaaggc ctttgcacag agctcctccc tcattgagca ccagaggatc
     1381 cacacgggag agaagcctta taagtgcagc gactgtggga aggccttcag tcgtagctca
     1441 gccctgatgg ttcacttgcg gatccacatc acggtgctgc aatagtaatg aactagtccg
     1501 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaaccata
     1561 cagctcgagg cagtaaa
//
