LOCUS       sequence-281309-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0642
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagtag ggaagatgag ctgaggaact gtgtggtatg tggggaccaa
       61 gccacaggct accactttaa tgcgctgact tgtgagggct gcaagggttt cttcaggaga
      121 acagtcagca aaagcattgg tcccacctgc ccctttgctg gaagctgtga agtcagcaag
      181 actcagaggc gccactgccc agcctgcagg ttgcagaagt gcttagatgc tggcatgagg
      241 aaagacatga tactgtcggc agaagccctg gcattgcggc gagcaaagca ggcccagcgg
      301 cgggcacagc aaacacctgt gcaactgagt aaggagcaag aagagctgat ccggacactc
      361 ctgggggccc acacccgcca catgggcacc atgtttgaac agtttgtgca gtttaggcct
      421 ccagctcatc tgttcatcca tcaccagccc ttgcccaccc tggcccctgt gctgcctctg
      481 gtcacacact tcgcagacat caacactttc atggtactgc aagtcatcaa gtttactaag
      541 gacctgcccg tcttccgttc cctgcccatt gaagaccaga tctcccttct caagggagca
      601 gctgtggaaa tctgtcacat cgtactcaat accactttct gtctccaaac acaaaacttc
      661 ctctgcgggc ctcttcgcta cacaattgaa gatggagccc gtgtggggtt ccaggtagag
      721 tttttggagt tgctctttca cttccatgga acactacgaa aactgcagct ccaagagcct
      781 gagtatgtgc tcttggctgc catggccctc ttctctcctg accgacctgg agttacccag
      841 agagatgaga ttgatcagct gcaagaggag atggcactga ctctgcaaag ctacatcaag
      901 ggccagcagc gaaggccccg ggatcggttt ctgtatgcga agttgctagg cctgctggct
      961 gagctccgga gcattaatga ggcctacggg taccaaatcc agcacatcca gggcctgtct
     1021 gccatgatgc cgctgctcca ggagatctgc agctagtaat gaactagtcc gtaacttgaa
     1081 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcctaa agaggttgag
     1141 tattggtc
//
