LOCUS       sequence-281324-        9095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0661
FEATURES             Location/Qualifiers
     source          1..9095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      351..360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagatcc aggaatgatg agtctttttg gcgaggatgg gaatattttc
       61 agtgaaggtc ttgaaggcct cggagaatgt ggttacccgg aaaatccagt aaatcctatg
      121 ggtcagcaaa tgccaataga ccaaggcttt gcctctttac agccatccct tcatcatcct
      181 tcaactaatc aaaatcaaac aaagctgaca cattttgatc actataatca gtatgaacaa
      241 caaaagatgc atctgatgga tcagccgaac agaatgatga gcaacacccc tgggaacgga
      301 ctcgcgtctc cgcactcgca gtatcacacc cctcccgttc ctcaggtgcc ccatggtggc
      361 agtggtggcg gtcagatggg tgtctaccct ggcatgcaga atgagaggca tgggcaatcc
      421 tttgtggaca gcagctccat gtggggcccc agggctgttc aggtaccaga ccagatacga
      481 gccccctacc agcagcagca gccacagccg cagccaccgc agccggctcc gtcggggccc
      541 cctgcacagg gccaccctca gcacatgcag cagatgggca gctatatggc acgtggggat
      601 ttttccatgc agcagcatgg tcagccacag cagaggatga gccagttttc ccaaggccaa
      661 gagggcctca atcagggaaa tccttttatt gccacctcag gacctggcca cttgtcccac
      721 gtgccccagc agagtcccag catggcacct tccttgcgtc actcggtgca gcagttccat
      781 caccacccct ctactgctct ccatggagaa tccgttgccc acagtcccag attctccccg
      841 aatcctcccc aacaaggggc tgttaggccg caaaccctta actttagttc tcggagccag
      901 acagtcccct ctcctactat aaacaactca gggcagtatt ctcgatatcc ttacagtaac
      961 ctaaatcagg gattagttaa caatacaggg atgaatcaaa atttaggcct tacaaataat
     1021 actccaatga atcagtccgt accaagatac cccaatgctg taggattccc atcaaacagt
     1081 ggtcaaggac taatgcacca gcagcccatc caccccagtg gctcacttaa ccaaatgaac
     1141 acacaaacta tgcatccttc acagcctcag ggaacttatg cctctccacc tcccatgtca
     1201 cccatgaaag caatgagtaa tccagcaggc actcctcctc cacaagtcag gccgggaagt
     1261 gctgggatac caatggaagt tggcagttat ccaaatatgc cccatcctca gccatctcac
     1321 cagccccctg gtgccatggg aatcggacag aggaatatgg gccccagaaa catgcagcag
     1381 tctcgtccat ttataggcat gtcctcggca ccaagggaat tgactgggca catgaggcca
     1441 aatggttgtc ctggtgttgg ccttggagac ccacaagcaa tccaggaacg actgatacct
     1501 ggccaacaac atcctggtca acagccatct tttcagcagt tgccaacctg tcctccactg
     1561 cagcctcacc cgggcttgca ccaccagtct tcacctccac accctcatca ccagccttgg
     1621 gcacagctcc acccatcacc ccagaacacc ccgcagaaag tgcctgtgca tcagcattcc
     1681 ccgtcggagc cctttctaga gaaaccagtg ccggatatga ctcaggttag tggaccgaat
     1741 gctcagctag tgaagagtga tgattacctg ccatcaatag aacagcagcc acaacaaaag
     1801 aagaagaaaa agaaaaacaa ccacattgta gcagaggatc ccagtaaagg ttttggtaaa
     1861 gatgacttcc ctggtggggt agataaccaa gaactaaata ggaactcact ggatgggtcc
     1921 caagaagaaa aaaagaaaaa gaaaaggtca aaggcaaaaa aagacccgaa ggaaccgaaa
     1981 gaacccaagg agaaaaaaga gcccaaggaa cccaagaccc cgaaagcccc taagattccc
     2041 aaagagccaa aggaaaagaa agcaaaaact gccacgccaa aacccaaatc cagcaaaaag
     2101 tcaagtaata agaaacctga ctcagaagca agtgctttga agaaaaaggt caacaaggga
     2161 aaaacagaag gttctgaaaa ttcagactta gacaaaacac ccccaccatc tcctcctcct
     2221 gaagaagatg aggacccagg tgttcagaag agacggtcca gcagacaggt gaagagaaag
     2281 cgctacactg aagacctgga gttcaagatt tctgatgagg aggcagatga tgcagatgct
     2341 gctgggaggg attccccctc caacacctcc cagtcagaac agcaggaatc tgttgatgca
     2401 gaaggcccag tggtagaaaa aattatgagc agtcgttcag taaaaaagca gaaggaatct
     2461 ggagaggagg tagaaattga ggaattctat gtgaaataca aaaacttctc ttatcttcat
     2521 tgtcagtggg catctataga agatctggaa aaagataaga gaattcagca aaaaattaaa
     2581 cgatttaagg caaagcaggg ccagaacaag ttcctttcag agattgagga tgagcttttt
     2641 aatccagatt atgtggaggt tgaccggata atggactttg cacgtagcac agatgaccgg
     2701 ggagagcctg tgactcacta tctggtgaag tggtgttcac ttccttatga agacagcacg
     2761 tgggagcgga ggcaggacat agatcaagca aagatcgagg agtttgagaa actaatgtcc
     2821 agggagccgg aaacagagcg tgtggagcga cctcctgctg atgattggaa gaaatcggag
     2881 agttccaggg agtataaaaa caataacaaa ctcagggaat accagttgga gggagtaaac
     2941 tggctacttt tcaattggta caacatgcga aactgcattt tagcagatga aatgggtttg
     3001 ggaaaaacta tccagtccat tacatttctc tatgagatat atttgaaagg aatccatggc
     3061 ccttttttag taattgcccc attgtccaca atccccaact gggaaaggga attccgaacc
     3121 tggacagagt tgaacgtggt tgtgtatcat gggagtcaag ctagtcgtcg gaccattcag
     3181 ttgtatgaaa tgtacttcaa agatccccag ggtcgagtga taaaggggtc ctataagttt
     3241 catgccatca tcactacatt tgagatgatt ttgactgatt gtcctgagct gcggaatatt
     3301 ccatggcgct gtgtagtcat tgatgaagcc cacaggctga agaacaggaa ctgcaagctg
     3361 ttggagggac tcaagatgat ggacttggaa cacaaagtgc tgctgacggg aaccccactc
     3421 cagaacactg tggaagaact cttcagcttg cttcatttct tggaaccaag tcgcttccct
     3481 tcagaaacca catttatgca agaatttggt gatctaaaaa cagaagagca ggtgcaaaaa
     3541 cttcaagcta ttctaaagcc aatgatgttg agacgtctca aagaggatgt agaaaagaac
     3601 ttggccccca aagaagaaac tattattgaa gttgagctaa caaacattca gaagaaatat
     3661 taccgagcca tccttgagaa gaatttcaca tttctttcca aaggcggtgg tcaagctaac
     3721 gtacctaacc tattaaacac tatgatggaa ttgcggaagt gctgcaatca tccgtacctt
     3781 atcaatggtg ctgaagagaa aattttggaa gagtttaaag aaacacacaa tgcagagtct
     3841 ccagattttc agctccaggc aatgatccag gctgctggca agctagtgct gattgacaag
     3901 ctgctgccaa aactgaaggc tggtggccac agggtgctta tcttttccca gatggtgcgc
     3961 tgcttggaca tactggaaga ctacctcatt caaagacggt acccatatga aaggatcgac
     4021 ggccgagtaa gaggcaacct ccgccaggca gctatcgaca gattctccaa acctgattct
     4081 gataggtttg ttttcctcct gtgtacaagg gcaggaggtt taggcattaa cctcactgct
     4141 gctgatacct gcatcatctt tgattcagac tggaatcccc aaaatgacct ccaggctcag
     4201 gctagatgtc atagaatagg acagagcaaa tctgtgaaaa tctacaggct gattacaaga
     4261 aattcctatg aaagggaaat gttcgacaag gctagtttga aactgggcct ggataaagct
     4321 gtgctacagt ctatgagtgg aagagaaaat gctaccaatg gggtacaaca gctttccaag
     4381 aaagaaatag aggatcttct acgaaaaggg gcctatggtg cactcatgga tgaggaggat
     4441 gaagggtcta aattctgtga agaagatatt gatcagatcc tcctacgtcg aacccacacc
     4501 attaccattg agtcagaagg gaaaggttcc acatttgcta aggccagttt tgttgcatct
     4561 ggaaatagga cagatatttc cttggatgat ccaaatttct ggcaaaagtg ggctaagaag
     4621 gctgaattgg atattgatgc cttaaatggg aggaacaacc tggttattga tactccaaga
     4681 gtgagaaagc agaccaggct ctacagtgca gtgaaggaag atgagctgat ggagttctca
     4741 gacttggaaa gtgattctga agaaaagccc tgtgcaaagc cacggcgtcc ccaggataag
     4801 tcacagggct atgcaaggag tgaatgtttc agggtggaga agaatctgct tgtctatggt
     4861 tggggacggt ggacagacat tctttcccac ggacgctata aacgccaact cactgagcaa
     4921 gatgtagaaa ccatctgcag aaccatcctg gtgtactgtc ttaatcatta caaaggggat
     4981 gagaatatca aaagcttcat ctgggatctg atcacaccca cagcggatgg ccagactcga
     5041 gccttggtca accattccgg tttgtcagct cctgtgccaa ggggaaggaa gggaaagaag
     5101 gtgaaagccc agagcacaca gccggtggtg caggatgccg actggctggc cagctgcaac
     5161 ccagatgccc tgttccagga ggacagctac aagaaacacc tgaagcatca ctgtaacaag
     5221 gtcctgctgc gtgtccgcat gctgtactac ctaagacaag aagtgatagg agaccaggcg
     5281 gataagatct tagagggtgc tgactcaagt gaagccgatg tgtggatccc tgaacctttc
     5341 catgctgaag ttcctgcaga ttggtgggat aaggaagcag acaaatccct cttaattgga
     5401 gtgttcaaac atggctatga gaagtacaac tccatgcgag ctgaccccgc gctgtgcttt
     5461 ctggaacgag tcggtatgcc tgatgccaag gccatagctg ccgagcaaag aggaacagac
     5521 atgctagcag atggtggtga cgggggagaa tttgatagag aagatgaaga cccagaatat
     5581 aaaccaacca gaacaccgtt caaagatgaa atagatgaat ttgcaaattc tccttcagag
     5641 gataaggaag aatccatgga aatacatgcc acaggcaagc acagtgagag taatgctgag
     5701 ttaggccaac tttactggcc taacacttca accctgacta cacgtctgcg ccggctcatt
     5761 actgcctatc agcgcagcta taaaaggcaa cagatgaggc aagaggccct aatgaagact
     5821 gaccggcgca gacggcggcc tcgagaggaa gtgagagctc tggaagcgga aagggaagct
     5881 attatatctg agaagcggca aaagtggaca agaagagaag aggctgattt ttaccgtgtg
     5941 gtatccacct ttggggttat ttttgaccct gtgaaacagc aatttgactg gaaccaattt
     6001 agagcctttg ccaggcttga caaaaaatct gatgagagtt tggagaaata cttcagttgt
     6061 tttgtggcca tgtgtaggcg agtatgtcga atgcccgtca agccagatga tgaaccgccc
     6121 gacctctcct ccataattga gccgatcaca gaggagcgag cctctcgaac tctgtaccgc
     6181 attgagctgc tacggaagat ccgcgagcag gttctccatc acccccagct gggagagagg
     6241 cttaagctct gccagccaag cttggatctg ccagagtggt gggagtgtgg acggcatgac
     6301 cgagacttgc tggttggtgc tgctaaacac ggggtcagtc ggacggatta tcacatcctc
     6361 aatgaccctg agttatcctt cttggatgca cataaaaact ttgctcaaaa cagaggggca
     6421 ggtaatacat cttccttgaa cccactggca gttggatttg tccagactcc tccagtcatc
     6481 tcatctgctc atattcaaga tgagagggta ctggaacaag ccgaaggcaa agtggaggag
     6541 cctgaaaacc cagctgccaa ggagaaatgt gagggcaaag aagaggaaga agaaaccgat
     6601 ggcagcggga aggagagcaa gcaggaatgt gaggcagagg ccagctctgt gaaaaatgaa
     6661 ctgaaaggtg ttgaggtcgg cgcagacact gggtccaaat ctatttcaga gaaaggttcc
     6721 gaagaggatg aagaggaaaa gctggaggat gacgataagt cggaagagtc ttcccagccc
     6781 gaagcaggag ctgtctctag agggaagaat tttgatgaag aaagcaatgc ttccatgagc
     6841 actgctagag atgaaacccg agatggattc tacatggagg acggagatcc ttcagtagct
     6901 cagctccttc atgaaagaac atttgccttc tcgttttggc ctaaggatag agtaatgata
     6961 aaccgcttag acaacatctg tgaagcagtg ttgaaaggca aatggccagt aaataggcgc
     7021 cagatgtttg atttccaagg cctcatccca ggttacacac ccaccacagt ggacagcccc
     7081 ttgcagaaga ggagctttgc tgagctctcc atggtcggcc aagccagcat tagtgggagt
     7141 gaggacatca ctacgtctcc tcagttgtca aaggaagatg ccctcaacct ctctgtccct
     7201 cgccagcgga ggaggaggag gagaaaaatc gaaattgagg ccgaaagagc tgccaagagg
     7261 cgaaatctca tggagatggt tgcccagctt cgagagtctc aggtggtctc agaaaatgga
     7321 caagaaaaag ttgtagattt atcaaaggcc tcaagagagg caacaagctc tacctcaaat
     7381 ttttcatctc tttcttcaaa gtttatcttg cctaatgtct caacaccagt gtctgatgcc
     7441 tttaagactc aaatggaact gctccaagca ggcctttcgc gcacacccac aaggcatctc
     7501 cttaatggct ccctagtgga tggagagcct cccatgaaga ggaggcgggg aaggaggaaa
     7561 aatgtggagg gacttgatct gcttttcatg agccacaaac ggacgtcatt gagtgcagag
     7621 gatgctgagg tgaccaaagc ttttgaagaa gatatagaga ccccaccaac aagaaacatt
     7681 ccttctcccg gacagctgga cccagacaca cggatccctg ttatcaatct tgaagatggg
     7741 actaggctgg tgggggaaga tgctcctaaa aataaggatt tagttgaatg gctgaagctg
     7801 caccctactt acactgttga tatgccaagt tatgtaccaa agaatgcaga tgtgctgttt
     7861 tcctcatttc agaaaccgaa acagaaacga catagatgtc gaaaccctaa taaattggat
     7921 ataaacactt tgacaggaga agaaagggtg cctgttgtca ataaacgaaa tgggaagaag
     7981 atgggtggag ctatggcgcc tccaatgaag gatctaccca ggtggctgga agaaaatcct
     8041 gaatttgcag ttgctccaga ctggactgat atagttaagc agtctggttt tgttcctgag
     8101 tcgatgtttg accgccttct cactgggcct gtagtgcggg gagagggagc gagcagaaga
     8161 ggaagaaggc ccaaaagtga gatcgccaga gcagccgcgg ccgccgctgc tgtggcctcc
     8221 acgtcaggga tcaacccttt gctggtgaac agcctgtttg ctggaatgga cctgacgagc
     8281 cttcagaatc tccagaatct ccagtcgctc cagctggcag gcctcatggg cttccctcca
     8341 ggactggcaa cagctgccac cgccggaggc gatgcgaaga accctgctgc tgtgctgccc
     8401 ctgatgctgc caggaatggc gggcctgccc aacgtgtttg gcttgggcgg gctgttgaat
     8461 aaccctctgt cagctgctac tggaaacacc actactgctt ctagtcaagg agaaccggaa
     8521 gacagcactt caaaaggaga ggagaaagga aatgagaatg aagacgagaa caaagactct
     8581 gagaaaagca cagatgctgt ttcggctgct gactctgcga atggatctgt tggtgctgct
     8641 actgccccgg ctggattgcc ctcaaacccg ctagccttca accctttcct cctgtccaca
     8701 atggccccgg gcctcttcta cccatccatg tttctacctc caggactggg gggattgacg
     8761 ctgcctgggt tcccagcatt ggcaggactt cagaatgccg tgggctccag cgaagaaaag
     8821 gctgctgaca aggctgaggg aggacccttt aaagatggag agacccttga aggcagcgat
     8881 gccgaggaga gcctggataa gactgcagag tcctccctct tagaagacga aatagcacag
     8941 ggtgaagagc tagactcact tgatgggggg gatgaaatag aaaacaatga aaatgatgaa
     9001 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     9061 ggaaaggacg aagaccctcc tcaacacaat actgt
//
