LOCUS       sequence-281327-        8249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0662
FEATURES             Location/Qualifiers
     source          1..8249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgaaaatgaa aatacagaaa aaagagaagc agttgtcaaa tttaaaagtt
       61 ttgaatcact ccccaatgtc tgatgcctct gtcaattttg actacaaatc tccatcccca
      121 tttgactgca gcactgatca agaagagaaa attgaagatg ttgctagtca ctgtctgcct
      181 cagaaggacc tgtatactgc tgaagaggaa gctgctaccc tttttcctag gaaaatgaca
      241 tcccataatg ggatggagga cagtggagga ggaggtactg gagtgaagaa gaaacggaag
      301 aaaaaggagc caggagacca agagggtgca gcaaagggaa gcaaggacag agagcccaag
      361 ccaaagagga aacgagaacc gaaagagcca aaggaaccca gaaaggccaa ggagccgaag
      421 aaggccaagg agcacaagga gccgaagcaa aaagatgggg caaagaaggc acggaagccc
      481 cgggaggcct cgggcaccaa ggaggccaaa gagaagagga gctgcactga ctctgcagcc
      541 aggacgaagt ccaggaaggc cagcaaggag caaggaccaa ccccagtgga gaaaaagaag
      601 aaaggaaaaa ggaaaagtga aactacagtg gagagtttag agctggatca gggcctgacg
      661 aacccatctc tgcggagtcc tgaggagtcc actgagtcta cagacagcca gaaacgacgc
      721 tcgggaaggc aagtaaagcg cagaaaatac aatgaggacc tggacttcaa agtggtggat
      781 gatgatgggg aaacaattgc tgttcttgga gctggtcgaa catctgcact ctcagcctct
      841 acactggcct ggcaggcgga ggagcctcca gaagatgatg caaacatcat tgagaagatc
      901 ctggcatcta agactgtcca ggaggttcac ccaggagaac ctccgttcga cttggagctg
      961 ttctacgtta agtatagaaa tttttcctac ttacattgta aatgggccac aatggaagag
     1021 ctcgaaaagg atcctcgcat cgcacagaag atcaagcgat ttaggaataa acaagcccag
     1081 atgaagcaca tttttacgga gcctgatgaa gacttgttca atccagacta tgtagaagtt
     1141 gatcgcatct tggaggtggc ccacaccaag gatgcagaaa caggggagga ggtaacacat
     1201 tacctggtga agtggtgctc actaccatat gaagaaagca cgtgggagct agaggaagat
     1261 gtagatcctg caaaagttaa agaatttgaa tctcttcaag ttctccctga aattaagcat
     1321 gtggagcggc ctgcttcaga ctcctggcag aaacttgaga agtctcgcga gtataagaac
     1381 agtaaccagc tccgggagta ccagctggaa gggatgaact ggcttctttt taactggtat
     1441 aacagaaaaa actgtatttt ggctgatgag atgggcctag ggaaaaccat ccagtccatc
     1501 acattccttt cagaaatatt tctgagagga atccacggcc cttttctcat tatcgcccct
     1561 ctctccacca tcactaactg ggagcgggag ttccggacat ggacagagat gaatgccatt
     1621 gtgtaccacg gcagccagat cagcaggcag atgatccagc agtatgaaat ggtgtacaga
     1681 gacgcccagg gaaaccccct ttcaggagtc ttcaagttcc acgtcgtcat cacaacattt
     1741 gaaatgatcc tagcagactg cccagagttg aagaagattc actggagctg tgtgataatt
     1801 gatgaagccc acagactgaa gaataggaac tgcaaacttc tggagggtct aaagcttatg
     1861 gccctggaac ataaagtgct tctcactgga acacccttgc agaactctgt ggaggagctc
     1921 ttcagtttgt taaattttct ggagccatca cagtttcctt cagagaccgc tttcttggag
     1981 gaatttggag atctgaaaac agaggagcag gtaaagaaac tgcagtctat cctaaaacca
     2041 atgatgcttc ggcggctgaa agatgatgtg gaaaagaacc ttgctcccaa acaagagacg
     2101 atcattgagg tggaactgac caatatccag aaaaagtact accgtgccat cctcgagaag
     2161 aacttttcct tcctgaccaa gggggcaaat cagcacaaca tgcccaatct catcaacacc
     2221 atgatggagc tgaggaagtg ctgtaaccat ccctacctga tcaatggagc agaggagaaa
     2281 attctagaag atttccgaaa aacccacagc cctgatgccc ctgactttca gctgcaggcc
     2341 atgattcagg cagcaggaaa gcttgtgttg attgataaac tactccctaa gctgattgca
     2401 ggtggccaca aagtactcat cttctcccag atggtgcgct gcctcgacat cctagaagat
     2461 tacctcatcc agagaagata cacctatgag cgaattgatg ggcgagtacg gggaaacctg
     2521 cgccaggcag ccatcgaccg gttctgtaag ccagattcag accgctttgt ctttcttctg
     2581 tgcaccagag cgggaggcct ggggatcaat ctcacagctg ctgatacctg catcatattt
     2641 gattctgact ggaacccaca aaatgacttg caggctcagg cccgatgtca ccgcataggc
     2701 cagagcaaag ctgtgaaggt gtatcgcctc atcactcgaa attcctacga gcgcgagatg
     2761 tttgacaagg ccagcctaaa gctggggctg gacaaggctg ttcttcagga catcaaccga
     2821 aagggcggca ccaatggggt acagcagctc tcaaaaatgg aggtggagga cctactccgg
     2881 aaaggtgctt atggagcctt aatggatgaa gaagatgaag gctccaagtt ctgtgaagaa
     2941 gacatagacc agattctgca gaggcgaacg cacaccatca ccatccagtc tgaggggaaa
     3001 gggtccactt ttgccaaggc tagctttgtg gcttcaggaa acagaacaga tatttcctta
     3061 gatgatccta acttttggca gaaatgggct aaaatagctg aactagacac tgaagcaaag
     3121 aatgaaaagg aaagcttagt gatcgaccga cctcgcgtga gaaagcagac caaacactac
     3181 aactcgtttg aggaagacga gctcatggag ttttcagagt tagacagcga ctcagacgaa
     3241 aggcccacga gatccaggcg cctcaatgac aaagccaggc gctacctccg agcggagtgc
     3301 ttccgggtag agaagaacct gctcatcttt ggctggggcc ggtggaagga catcctgact
     3361 catggccgat tcaagtggca tctgaacgag aaggacatgg agatgatttg ccgtgccctc
     3421 ctggtgtact gtgtcaagca ttataagggg gacgagaaga tcaagagttt catttgggaa
     3481 ctgatcacac ctaccaaaga tgggcaagcc cagaccctcc agaaccactc aggcttatct
     3541 gccccagtcc ccagagggag gaaggggaag aagacgaaga accagttgct aatcccagag
     3601 ctaaaggatg cagattggct agccacctgc aaccccgagg tggtcttgca tgatgatggc
     3661 tataagaaac acctcaaaca gcactgcaac aaagtacttt tgcgagtccg gatgctgtac
     3721 tacctaaaag ctgaaatact gggagaagca gctgagaaag catttgaagg atctcctgcc
     3781 agggagctgg atgtacctct gcctgacatc gactacatgg agatcccagt ggactggtgg
     3841 gatgctgaag ccgataagtc acttctcatt ggcgtgttca agcatggtta tgaaaggtac
     3901 aatgccatgc gagcagaccc agcactttgc ttcctggaga aagttgggat gcccgatgag
     3961 aagtcccttt ctgcagaaca gggtgttaca gatgggacct cagacattcc tgaaagaggc
     4021 aacacagata aagaagacaa tgctgaggac aaagtagatg gcctccagaa acaaacggag
     4081 agttccagtg atggaggtga tggcgttttt agcgaaaaga aggatgacag ccgggcagcc
     4141 caggatggct ccgacccaga caaatcgccc tggccagttt cctccgccct cacagctcgt
     4201 ctcagacgtc tggtcactgt ttaccagcgc tgcaaccgca aggaactgtg ccggcctgaa
     4261 attctgggac caggtaacca aggatattgg gttcaggaag agatgttcag gagaacctca
     4321 gaaatggacc tcatcaacaa ggaagcccaa aagaggtgga ctaggagaga acaagcagac
     4381 ttctatagaa cagtgtcttc ctttggtgtt gtttacgatc aagaaaagaa aacctttgac
     4441 tggacacagt tccgcatcat ttcccgtttg gacaagaagt cggatgagag cctggaacag
     4501 tatttttata gttttgtggc catgtgccgg aatgtctgtc gtctacccac atggaaagat
     4561 ggcggtcccc cagataccac catctacgtt gaacccatca ctgaggaacg tgctgcaaga
     4621 actctgtacc gcattgaact gttacggaaa gtccgagagc aagtgctcaa gtgccctcag
     4681 ctgcatgaac gcctccagct gtgcaggccc agcctctacc tcccagtctg gtgggagtgt
     4741 gggaagcatg atcgagacct gctcatcggc actgccaaac atgggctgaa ccgcactgac
     4801 tgttacatca tgaacgaccc ccagctgtcc ttcctggatg cctatagaaa ctatgcccag
     4861 cataaaagat ctggcaccca ggcaccagga aatctctgtt gcctttacca gaccaactcc
     4921 aagttatatg aatctcttac atattctcaa atgagtagga cttcagagtc ccttgaaaat
     4981 gaacctgaaa atctagtgag agtagaaagc agagatgatc atctcagcct gcctgatgtg
     5041 acatgtgaaa actttatttc taaagttcag gatgtcattt ccatcaacca tgatgaaagt
     5101 ctgctgcctg agtccttaga gagcatgatg tatggtaaga aggtgctcag ccaagaacca
     5161 agctcttttc aggagagccc aagtaccaat actgaatcta gaaaagatgt tattaccatc
     5221 tcaataagca aagatgggaa ctgccagtct ggtggccctg aggcagaaat agcttctggc
     5281 cctactttta tgggtagctt agaagcagga ggagtagctc aagcaaacat caaaaatgga
     5341 aaacatttgt tgatgtctat ttcaaaggaa ggggagctct gctgcagtga ggcaggacag
     5401 agacctgaaa acattggcca gctggaagcc aagtgtttag cttccccttc cttgaatcca
     5461 ggaaatgaaa gtgggtttgt agatatgtgc agtcttagtg tctgtgactc caaaagaaac
     5521 ctgtcatcag atcagcaatt aattgattta ttggaaaaca aaagcttaga aagtaaattg
     5581 attttgagtc agaaccacag tgatgaggag gaagaagagg aggaaaacga ggaggaaaac
     5641 ttagccatgg cagtaggcat gggggaaagg ccagaggtat tgcatctcac ggagcccact
     5701 actaacatct caagggaaaa gaaccaaggc ttccaagatg aaaccaagaa aggaagctta
     5761 gaggtggcaa accagactcc tgggctacag agggctttcc ccgctccagc agcctgtcag
     5821 tgccactgca aacacatgga gaggtggatg catggcctcg agaatgatga atttgaaatc
     5881 gagaaaccca aggcttatat cccagatctg ttcaaaagta aaaccaatac tatcgccatg
     5941 gagggtgaac ccactgctat tccatcacag ccgtttaaag tgaagcatga gcttttaaaa
     6001 gaaccttgga aagaaagtgc agaggggcaa aacgttttcc ccacatatcc tcttgaagga
     6061 agtgagctca aatcagaaga catggatttt gagaataaag atgattatga tagagacgga
     6121 aactgccata gtcaagatta tccagggaag tactctgaag aggagagcaa gagctcaaca
     6181 tcgggcatca caggagacat tggggatgag ctacaggagg ctcgagctcc cactattgct
     6241 cagctgctac aggagaaaac tctctattcc ttctctgagt ggccaaagga ccgcgtgata
     6301 attaaccgcc tagataatat ctgccacgtg gtgttaaagg ggaagtggcc ctctagccag
     6361 cagtatgagc cctcaggcac actgcccacc ccggtattaa ccagcagtgc tggttctcga
     6421 accagcctct cagagccgga agcagcagaa cacagcttca gcaacggcgc agcattggcg
     6481 gcccagatcc acaaggagag cttcttagct ccagtattca caaaggatga acaaaagcac
     6541 aggcgtccct atgagtttga ggtggagagg gatgcaaagg ctcggggcct ggagcagttc
     6601 tctgccaccc acgggcacac ccctatcatc ctcaatggct ggcatgggga gtcagctatg
     6661 gacctctcct gctcatcaga ggggtcccca ggagccacat cccctttccc agtgagcgcc
     6721 agcaccccta agattggggc tatcagttca cttcagggag cccttggcat ggacttgtct
     6781 gggattctgc aagctggcct gatccatcct gtgactggac agattgtcaa tggaagcctc
     6841 agaagagatg atgcagccac gaggaggcgg agagggaggc ggaaacatgt tgaaggaggg
     6901 atggacctca tctttttgaa ggagcagaca cttcaggcgg gaatcttgga agtccatgaa
     6961 gacccagggc aggccacctt gagcaccaca caccctgagg ggccagggcc tgccacctcg
     7021 gctcctgagc cagctacggc agccagcagc caagccgaga aatccattcc cagcaagagt
     7081 ctgcttgact ggctaaggca gcaggctgac tactccttag aagttcctgg ctttggggca
     7141 aatttttcag acaaaccaaa gcagaggagg ccacgctgta aagaacctgg aaaattagat
     7201 gtcagctccc tgagcgggga agagagagtt cctgccatcc ccaaggagcc aggactgagg
     7261 gggtttcttc cagaaaacaa gttcaatcac actctggctg agcctattct tcgagatacg
     7321 ggcccccgca ggagggggag gcggcctcgg agcgaactcc tgaaggctcc ttccattgtg
     7381 gcagactctc cctctggaat ggggccactg ttcatgaatg gactgattgc tgggatggac
     7441 ctggtaggac ttcagaacat gagaaatatg ccaggcatcc ccctcaccgg gctggtgggg
     7501 tttccagctg gctttgccac gatgccaaca ggtgaagagg tcaaaagtac cctgagcatg
     7561 ctgcccatga tgctgccagg catggctgct gtgccccaga tgtttggtgt tgggggactc
     7621 ctcagtccac ccatggcaac cacctgcact tccactgctc cggcgtctct atcaagcaca
     7681 acgaaaagtg gtacggcagt gactgaaaag actgcggaag acaagccgag tagccatgat
     7741 gtgaaaacag acactttagc tgaggacaag cctggtccag gtccattttc tgatcagtct
     7801 gaacctgcaa taactactag tagtcctgtg gcttttaacc catttctcat cccaggagta
     7861 tctcctggac tcatttaccc atccatgttc ctctcccctg gtatgggcat ggctctgcca
     7921 gccatgcagc aggccagaca ctcggaaata gtaggtctgg agagccagaa gaggaagaag
     7981 aagaagacaa agggggacaa ccccaactcc cacccagagc ctgctcccag ctgtgaaagg
     8041 gagcccagcg gtgatgagaa ctgtgccgaa cccagtgccc ctttgcccgc agagagagaa
     8101 catggggcac aggctgggga gggggcactc aaagactcca acaacgacac caattagtaa
     8161 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     8221 gacgatcagt tctgccgggt cacgtctcg
//
