LOCUS       sequence-281330-        1430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0692
FEATURES             Location/Qualifiers
     source          1..1430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtcagcgt gaacgcgccc ctcggggctc cagtggagag ttcttacgac
       61 acgtccccat cagaaggcac caacctcaac gcgcccaaca gcctgggtgt cagcgccctg
      121 tgtgccatct gcggggaccg ggccacgggc aaacactacg gtgcctcgag ctgtgacggc
      181 tgcaagggct tcttccggag gagcgtgcgg aagaaccaca tgtactcctg cagatttagc
      241 cggcagtgcg tggtggacaa agacaagagg aaccagtgcc gctactgcag gctcaagaaa
      301 tgcttccggg ctggcatgaa gaaggaagcc gtccagaatg agcgggaccg gatcagcact
      361 cgaaggtcaa gctatgagga cagcagcctg ccctccatca atgcgctcct gcaggcggag
      421 gtcctgtccc gacagatcac ctcccccgtc tccgggatca acggcgacat tcgggcgaag
      481 aagattgcca gcatcgcaga tgtgtgtgag tccatgaagg agcagctgct ggttctcgtt
      541 gagtgggcca agtacatccc agctttctgc gagctccccc tggacgacca ggtggccctg
      601 ctcagagccc atgctggcga gcacctgctg ctcggagcca ccaagagatc catggtgttc
      661 aaggacgtgc tgctcctagg caatgactac attgtccctc ggcactgccc ggagctggcg
      721 gagatgagcc gggtgtccat acgcatcctt gacgagctgg tgctgccctt ccaggagctg
      781 cagatcgatg acaatgagta tgcctacctc aaagccatca tcttctttga cccagatgcc
      841 aaggggctga gcgatccagg gaagatcaag cggctgcgtt cccaggtgca ggtgagcttg
      901 gaggactaca tcaacgaccg ccagtatgac tcgcgtggcc gctttggaga gctgctgctg
      961 ctgctgccca ccttgcagag catcacctgg cagatgatcg agcagatcca gttcatcaag
     1021 ctcttcggca tggccaagat tgacaacctg ttgcaggaga tgctgctggg agggtccccc
     1081 agcgatgcac cccatgccca ccaccccctg caccctcacc tgatgcagga acatatggga
     1141 accaacgtca tcgttgccaa cacaatgccc actcacctca gcaacggaca gatgtccacc
     1201 cctgagaccc cacagccctc accgccaggt ggctcagggt ctgagcccta taagctcctg
     1261 ccgggagccg tcgccacaat cgtcaagccc ctctctgcca tcccccagcc gaccatcacc
     1321 aagcaggaag ttatctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1381 ctttatatat cttgtggaaa ggacgatcac gcgctgacag gcctattgca
//
