LOCUS       sequence-281339-        1688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0712
FEATURES             Location/Qualifiers
     source          1..1688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gcaatttaga gatgtggcca tagaattctc tctggaggag
       61 tggcattgcc tggacactgc acagcggaat ttatatagga atgtgatgtt agagaactac
      121 agtaacctgg tcttccttgg tattactgtt tctaagccag acctgatcac ctgtctggag
      181 caaggaagaa aacctttgac catgaagaga aatgagatga tagccaaacc ctcagtaatg
      241 tgttctcatt ttgcccaaga cctttggcca gagcagagca tgaaagattc tttccaaaaa
      301 gtggtactga gaagatatga aaaatgtgaa catgacaatt tacagttaaa aaaaggatgt
      361 ataagtgtgg atgagtgtaa ggtgcacaaa gaaggttata atgaacttaa ccaatgtttg
      421 acaactaccc caagaaaaat atgtcaatgt gataaatatg tgaaagtcct tcatcaattt
      481 ccaaattcaa acggacaaaa gagaggacat actgggaaaa aacctttcaa atatatagaa
      541 tgtggcaaag cttttaagca gttctcaact cttactacac ataagaaaat tcatactgga
      601 gggaaaccct acaaatgtga agaatgtggc aaagccttta accactcttg tagccttact
      661 agacataaga aaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagcc
      721 tttaagcact cctccactct tactacacat aagagaaatc atactggaga gaaaccctac
      781 aagtgtgata aatgtggcaa agcctttatg tcatcctcaa cccttagtaa acatgagata
      841 attcatacgg aaaagaaacc ctacaaatgt gaagaatgtg gcaaagcctt caaccggtcc
      901 tcaaccctta ctacacataa gataattcat actggagaaa aaccctacaa atgtgaagaa
      961 tgtgacaaag cctttaagta ctcctatacc cttactacac ataaaagaat tcatactgaa
     1021 gacaaaccct acaaatgtga agaatgtggc aaagccttta agtactcctc tacccttact
     1081 acacataaga gaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagcc
     1141 ttcaagcggt cttcagacct tactacgcat aagataattc atactggaga gaaaccctac
     1201 aaatgtgaag aatgtggcaa agcttttaag tactcctcta accttactac acataagaaa
     1261 attcatactg gagagagacc ctacaaatgt gaagaatgtg gcaaagcctt caaccagtcc
     1321 tcaatcctta ctacacatag gagaattcat actggagaga aattctacaa atgtgaagaa
     1381 tgtggcaaag cttttaagtg ctcctctaac cttactacac ataagaaaat tcatactgga
     1441 gagagaccct acaaatgtga agaatgtggc aaagccttca accagtcctc aatccttact
     1501 acacatgaga gaatcatact ggagagaaat tctacaaatg tgaagaatgt ggcaaagcct
     1561 tcaagcggtc ctcacacctt actacacata agatagtaat gaactagtcc gtaacttgaa
     1621 agtatttcga tttcttggct ttatatatct tgtggaaagg acgataaact aaggaccatg
     1681 tagtatag
//
