LOCUS       sequence-28134-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, 
            Bartel DP
  TITLE     RNAi in budding yeast.
  JOURNAL   Science. 2009 Oct 23;326(5952):544-550. doi: 
            10.1126/science.1176945. Epub 2009 Sep 10.
  PUBMED    19745116
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1176945"; journalName: "Science"; date: 
            "2009-10-23- 23"; volume: "326"; issue: "5952"; pages: "544-550"
COMMENT     Sequence Label: pYES2.1-GFP
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..22)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(32..73)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(158..177)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          160..389
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             complement(475..726)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(517..538)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     866..885
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctcaatggtg atggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag
       61 ggataggctt accttcgaat gggtgacctc gaagctcgcc cttggaattg gccgccctag
      121 atgcatgctc gacctgcagt tggacctggg agtggacacc tgtggagaga aaggcaaagt
      181 ggatgtcatt gtcactcaag tgtatggcca gatctcaagc ctgccacacc tcaagcttga
      241 caacaaaaag attgtctttt ctgaccagat ggacgcggcc accctcaaag gcatcaccgc
      301 gggccaggtg aatatcaaat cctcctcgtt tttggaaact gacaatctta gcgcagaagt
      361 catgcccgct tttgagaggg agtactcacc ccaacagctg gccctcgcag acagcgaatt
      421 aattccagca cactggcggc cgttactagt ggatccactg aattcttaaa gcttcttgta
      481 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      541 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
      601 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      661 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
      721 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      781 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      841 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      901 gcgctcctgg acgtagcctt cgggcatggc gganttgaag aagtcgtgct gcttcatgtg
      961 gt
//
