LOCUS       sequence-281348-        2801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0724
FEATURES             Location/Qualifiers
     source          1..2801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagagttg ctacaaccca ggtctggatg gtattattga atatgatgat
       61 ttcaaattga actcctccat tgtggaaccc aaggagccag ccccagaaac agctgatggc
      121 ccctacctgg tgatcgtgga acagcctaag cagagaggct tccgatttcg atatggctgt
      181 gaaggcccct cccatggagg actgcccggt gcctccagtg agaagggccg aaagacctat
      241 cccactgtca agatctgtaa ctacgaggga ccagccaaga tcgaggtgga cctggtaaca
      301 cacagtgacc cacctcgtgc tcatgcccac agtctggtgg gcaagcaatg ctcggagctg
      361 gggatctgcg ccgtttctgt ggggcccaag gacatgactg cccaatttaa caacctgggt
      421 gtcctgcatg tgactaagaa gaacatgatg gggactatga tacaaaaact tcagaggcag
      481 cggctccgct ctaggcccca gggccttacg gaggccgagc agcgggagct ggagcaagag
      541 gccaaagaac tgaagaaggt gatggatctg agtatagtgc ggctgcgctt ctctgccttc
      601 cttagagcca gtgatggctc cttctccctg cccctgaagc cagtcatctc ccagcccatc
      661 catgacagca aatctccggg ggcatcaaac ctgaagattt ctcgaatgga caagacagca
      721 ggctctgtgc ggggtggaga tgaagtttat ctgctttgtg acaaggtgca gaaagatgac
      781 attgaggttc ggttctatga ggatgatgag aatggatggc aggcctttgg ggacttctct
      841 cccacagatg tgcataaaca gtatgccatt gtgttccgga caccccccta tcacaagatg
      901 aagattgagc ggcctgtaac agtgtttctg caactgaaac gcaagcgagg aggggacgtg
      961 tctgattcca aacagttcac ctattaccct ctggtggaag acaaggaaga ggtgcagcgg
     1021 aagcggagga aggccttgcc caccttctcc cagcccttcg ggggtggctc ccacatgggt
     1081 ggaggctctg ggggtgcagc cgggggctac ggaggagctg gaggaggtgg cagcctcggt
     1141 ttcttcccct cctccctggc ctacagcccc taccagtccg gcgcgggccc catgggctgc
     1201 tacccgggag gcgggggcgg ggcgcagatg gccgccacgg tgcccagcag ggactccggg
     1261 gaggaagccg cggagccaag cgccccctcc aggacccccc agtgcgagcc gcaggccccg
     1321 gagatgctgc agcgagctcg agagtacaac gcgcgcctgt tcggcctggc gcagcgcagc
     1381 gcccgagccc tactcgacta cggcgtcacc gcggacgcgc gcgcgctgct ggcgggacag
     1441 cgccacctgc tgacggcgca ggacgagaac ggagacacac cactgcacct agccatcatc
     1501 cacgggcaga ccagtgtcat tgagcagata gtctatgtca tccaccacgc ccaggacctc
     1561 ggcgttgtca acctcaccaa ccacctgcac cagacgcccc tgcacctggc ggtgatcacg
     1621 gggcagacga gtgtggtgag ctttctgctg cgggtaggtg cagacccagc tctgctggat
     1681 cggcatggag actcagccat gcatctggcg ctgcgggcag gcgctggtgc tcctgagctg
     1741 ctgcgtgcac tgcttcagag tggagctcct gctgtgcccc agctgttgca tatgcctgac
     1801 tttgagggac tgtatccagt acacctggca gtccgagccc gaagccctga gtgcctggat
     1861 ctgctggtgg acagtggggc tgaagtggag gccacagagc ggcagggggg acgaacagcc
     1921 ttgcatctag ccacagagat ggaggagctg gggttggtca cccatctggt caccaagctc
     1981 cgggccaacg tgaacgctcg cacctttgcg ggaaacacac ccctgcacct ggcagctgga
     2041 ctggggtacc cgaccctcac ccgcctcctt ctgaaggctg gtgctgacat ccatgctgaa
     2101 aacgaggagc ccctgtgccc actgccttca ccccctacct ctgatagcga ctcggactct
     2161 gaagggcctg agaaggacac ccgaagcagc ttccggggcc acacgcctct tgacctcact
     2221 tgcagcacca aggtgaagac cttgctgcta aatgctgctc agaacaccat ggagccaccc
     2281 ctgaccccgc ccagcccagc agggccggga ctgtcacttg gtgatacagc tctgcagaac
     2341 ctggagcagc tgctagacgg gccagaagcc cagggcagct gggcagagct ggcagagcgt
     2401 ctggggctgc gcagcctggt agacacgtac cgacagacaa cctcacccag tggcagcctc
     2461 ctgcgcagct acgagctggc tggcggggac ctggcaggtc tactggaggc cctgtctgac
     2521 atgggcctag aggagggagt gaggctgctg aggggtccag aaacccgaga caagctgccc
     2581 agcacagagg tgaaggaaga cagtgcgtac gggagccagt cagtggagca ggaggcagag
     2641 aagctgggcc caccccctga gccaccagga gggctctgcc acgggcaccc ccagcctcag
     2701 gtgcactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2761 tcttgtggaa aggacgacta tggaaccagg gcgccgacgt g
//
