LOCUS       sequence-28136-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, 
            Bartel DP
  TITLE     RNAi in budding yeast.
  JOURNAL   Science. 2009 Oct 23;326(5952):544-550. doi: 
            10.1126/science.1176945. Epub 2009 Sep 10.
  PUBMED    19745116
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1176945"; journalName: "Science"; date: 
            "2009-10-23- 23"; volume: "326"; issue: "5952"; pages: "544-550"
COMMENT     Sequence Label: pYES2.1-GFP
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..69)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(70..93)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(115..134)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(116..134)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(166..607)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(172..195)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    490..607
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(716..735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccttgctc accatggatc cgcccgggct agagcggccg ctagcgtaat cgggcacgtc
       61 ataagggtac ttgtcatcgt cgtccttgta gtccataagg gcgagcttaa tattccctat
      121 agtgagtcgt attacagctg ctagtagtcc gatccggggt tttttctcct tgacgttaaa
      181 gtatagaggt atattaacaa ttttttgttg atacttttat tacatttgaa taagaagtaa
      241 tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagttttt gcatttatat
      301 atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
      361 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
      421 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
      481 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
      541 agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct cggcggcttc
      601 taatccgtgg actagtggat cgatccccac gcgccctgta gcggcgcatt aagcgcggcg
      661 ggtgtggtgg ttacgcgcag cgngaccgct acacttgcca gcgccctagc gcccgctcct
      721 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      781 cgggggctcc ctttagggtt ccgatttagt nntttac
//
