LOCUS       sequence-281375-        1529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0896
FEATURES             Location/Qualifiers
     source          1..1529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgaggg atcagtgatg ttcagtgatg tgtccataga cttctctcag
       61 gaggagtggg actgcctgga ccctgttcag agggacttat acagagatgt gatgttggag
      121 aactacggca atctggtttc aatgggactt tacactccta agcctcaagt gatctcctta
      181 ttggaacaag ggaaagagcc ctggatggtt ggcagagagc ttacaagagg cctgtgttca
      241 gatctggaat cgatgtgtga aaccaagtta ttatctctaa agaaggaagt ttatgaaata
      301 gaattatgcc agagggagat aatgggactt acaaagcatg gccttgagta ctccagtttt
      361 ggagatgttt tggaatatag aagccacctt gcaaaacaac tgggatatcc aaatgggcat
      421 tttagtcaag aaatattcac tcctgaatac atgcccacat ttattcaaca gacattcctt
      481 actctccatc aaataattaa taatgaagac agaccctatg aatgtaagaa atgtggaaag
      541 gcctttagtc agaactcaca atttattcaa catcagagaa ttcatattgg tgaaaaatct
      601 tatgaatgta aagagtgtgg gaaattcttt agttgtggtt cacatgttac tcggcatctg
      661 aaaattcata ctggcgaaaa accctttgaa tgtaaggaat gtggaaaggc cttcagttgt
      721 agctcatacc tttctcaaca tcagagaatc cataccggta agaaacccta tgaatgtaag
      781 gaatgtggga aggcctttag ttattgctca aatcttattg accatcagcg aattcacact
      841 ggtgaaaaac cttatgaatg taaagtatgt gggaaagcct ttactaagag ctcacaactt
      901 tttcagcatg cacgaattca tacaggtgag aaaccctatg aatgtaagga atgtggcaaa
      961 gcctttaccc agagctcaaa gcttgttcaa catcagagaa ttcatactgg tgagaaaccc
     1021 tatgagtgca aggaatgtgg caaagccttt agtagtggct cagcacttac taatcatcag
     1081 agaattcaca ctggtgagaa accctatgat tgtaaggaat gtggaaaggc ttttactcag
     1141 agctcacagc ttcgtcaaca tcagagaatt cacgctggtg agaaaccctt tgaatgtctt
     1201 gaatgtggga aggcctttac tcagaactca caacttttcc agcatcagag aattcataca
     1261 gatgaaaaac catatgaatg taatgaatgt ggaaaggcct ttaataaatg ctcaaacctt
     1321 actcgacatc tgagaattca cactggtgaa aagccctata actgtaagga atgtgggaag
     1381 gcttttagta gtggctcgga tctcattcgt catcagggaa ttcatactaa taaatagtaa
     1441 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1501 gacgaggtgg aataaatgag ggtcttgtt
//
