LOCUS       sequence-28139-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, 
            Bartel DP
  TITLE     RNAi in budding yeast.
  JOURNAL   Science. 2009 Oct 23;326(5952):544-550. doi: 
            10.1126/science.1176945. Epub 2009 Sep 10.
  PUBMED    19745116
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1176945"; journalName: "Science"; date: 
            "2009-10-23- 23"; volume: "326"; issue: "5952"; pages: "544-550"
COMMENT     Sequence Label: plp
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      54..301
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(70..88)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(378..395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(629..648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actactagca gctgaaactc gagcctgcag gcggccgcat ctagagggcc gcatcatgta
       61 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
      121 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
      181 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
      241 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
      301 caagctgcgg ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      361 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      661 ctcgtgcgct ctcctgttcc gaccctgccg ctta
//
