LOCUS       sequence-281393-        1265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0963
FEATURES             Location/Qualifiers
     source          1..1265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgggcag cccagacacc gttgggatga actacggcag ctacatggag
       61 gagaagcaca tgccaccccc aaacatgacc acgaacgagc gcagagttat cgtgccagca
      121 gatcctacgc tatggagtac agaccatgtg cggcagtggc tggagtgggc ggtgaaagaa
      181 tatggccttc cagacgtcaa catcttgtta ttccagaaca tcgatgggaa ggaactgtgc
      241 aagatgacca aggacgactt ccagaggctc acccccagct acaacgccga catccttctc
      301 tcacatctcc actacctcag agagactcct cttccacatt tgacttcaga tgatgttgat
      361 aaagccttac aaaactctcc acggttaatg catgctagaa acacaggggg tgcagctttt
      421 attttcccaa atacttcagt atatcctgaa gctacgcaaa gaattacaac taggccagat
      481 ttaccatatg agccccccag gagatcagcc tggaccggtc acggccaccc cacgccccag
      541 tcgaaagctg ctcaaccatc tccttccaca gtgcccaaaa ctgaagacca gcgtcctcag
      601 ttagatcctt atcagattct tggaccaaca agtagccgcc ttgcaaatcc aggcagtggc
      661 cagatccagc tttggcagtt cctcctggag ctcctgtcgg acagctccaa ctccagctgc
      721 atcacctggg aaggcaccaa cggggagttc aagatgacgg atcccgacga ggtggcccgg
      781 cgctggggag agcggaagag caaacccaac atgaactacg ataagctcag ccgcgccctc
      841 cgttactact atgacaagaa catcatgacc aaggtccatg ggaagcgcta cgcctacaag
      901 ttcgacttcc acgggatcgc ccaggccctc cagccccacc ccccggagtc atctctgtac
      961 aagtacccct cagacctccc gtacatgggc tcctatcacg cccacccaca gaagatgaac
     1021 tttgtggcgc cccaccctcc agccctcccc gtgacatctt ccagtttttt tgctgcccca
     1081 aacccatact ggaattcacc aactgggggt atatacccca acactaggct ccccaccagc
     1141 catatgcctt ctcatctggg cacttactac tagtaatgaa ctagtccgta acttgaaagt
     1201 atttcgattt cttggcttta tatatcttgt ggaaaggacg agggcgtcaa gctgttctgg
     1261 atgct
//
