LOCUS       sequence-281396-        1469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0964
FEATURES             Location/Qualifiers
     source          1..1469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagcac tattaaggaa gccttatcag ttgtgagtga ggaccagtcg
       61 ttgtttgagt gtgcctacgg aacgccacac ctggctaaga cagagatgac cgcgtcctcc
      121 tccagcgact atggacagac ttccaagatg agcccacgcg tccctcagca ggattggctg
      181 tctcaacccc cagccagggt caccatcaaa atggaatgta accctagcca ggtgaatggc
      241 tcaaggaact ctcctgatga atgcagtgtg gccaaaggcg ggaagatggt gggcagccca
      301 gacaccgttg ggatgaacta cggcagctac atggaggaga agcacatgcc acccccaaac
      361 atgaccacga acgagcgcag agttatcgtg ccagcagatc ctacgctatg gagtacagac
      421 catgtgcggc agtggctgga gtgggcggtg aaagaatatg gccttccaga cgtcaacatc
      481 ttgttattcc agaacatcga tgggaaggaa ctgtgcaaga tgaccaagga cgacttccag
      541 aggctcaccc ccagctacaa cgccgacatc cttctctcac atctccacta cctcagagag
      601 actcctcttc cacatttgac ttcagatgat gttgataaag ccttacaaaa ctctccacgg
      661 ttaatgcatg ctagaaacac agatttacca tatgagcccc ccaggagatc agcctggacc
      721 ggtcacggcc accccacgcc ccagtcgaaa gctgctcaac catctccttc cacagtgccc
      781 aaaactgaag accagcgtcc tcagttagat ccttatcaga ttcttggacc aacaagtagc
      841 cgccttgcaa atccaggcag tggccagatc cagctttggc agttcctcct ggagctcctg
      901 tcggacagct ccaactccag ctgcatcacc tgggaaggca ccaacgggga gttcaagatg
      961 acggatcccg acgaggtggc ccggcgctgg ggagagcgga agagcaaacc caacatgaac
     1021 tacgataagc tcagccgcgc cctccgttac tactatgaca agaacatcat gaccaaggtc
     1081 catgggaagc gctacgccta caagttcgac ttccacggga tcgcccaggc cctccagccc
     1141 caccccccgg agtcatctct gtacaagtac ccctcagacc tcccgtacat gggctcctat
     1201 cacgcccacc cacagaagat gaactttgtg gcgccccacc ctccagccct ccccgtgaca
     1261 tcttccagtt tttttgctgc cccaaaccca tactggaatt caccaactgg gggtatatac
     1321 cccaacacta ggctccccac cagccatatg ccttctcatc tgggcactta ctactagtaa
     1381 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1441 gacgattaca taggctttcc gcaggagtc
//
