LOCUS       sequence-281405-        1964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1003
FEATURES             Location/Qualifiers
     source          1..1964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgcctgc tgagacttct tcatctggcc ttttggaaga gcaaaaaatg
       61 atgaagtccc aggggttagt atcattcaag gatgtggctg tggatttcac ccaggaggag
      121 tggcagcaac ttgacccttc tcagaggacc ctgtacaggg atgtgatgct ggagaactac
      181 agccacctgg tctcaatggg gtatccagtt tccaaaccag atgtcatctc caagttggaa
      241 caaggagaag agccatggat cataaaggga gacatatcaa attggatcta tccagatgaa
      301 tatcaggcag atgggagaca agacaggaag agtaaccttc acaactccca gtcatgtatt
      361 ttggggacag tttccttcca tcataagata ctgaaaggag tcacaaggga tggttcattg
      421 tgctccattt taaaagtctg tcaaggtgat ggtcagctgc agagatttct agagaatcaa
      481 gacaaactct tcaggcaggt cacatttgtt aacagcaaaa cagtgactga ggcatcaggg
      541 cataaatata atccactggg gaaaatattt caagagtgca tagaaacaga tatatcaata
      601 cagagattcc ataaatatga tgcttttaaa aagaacttaa aaccaaatat tgacctaccg
      661 agttgttata agagcaattc aagaaaaaaa cctgatcaga gttttggagg tggaaaatca
      721 tctagccaga gtgagcccaa ttctaatctt gagaagattc acaatggagt aatacctttt
      781 gatgataatc agtgtggaaa cgtttttaga aatacacaat cccttattca atatcagaat
      841 gtggaaacta aagagaaaag ctgtgtatgt gttacatgtg gaaaagcctt tgctaagaag
      901 tcacaactca ttgtacatca aagaattcat actggaaaga aaccatatga ttgtggtgca
      961 tgcggaaaag ccttcagtga gaagtttcat cttgttgtac atcagagaac tcatactggg
     1021 gagaaacctt atgattgttc tgaatgtgga aaagccttct ctcagaaatc gtcccttatt
     1081 atacatcaga gagttcacac tggggaaaaa ccctatgaat gtagtgaatg cgggaaagcc
     1141 ttctcccaga aatcacccct cattatacat cagagaatac atactgggga aaaaccctat
     1201 gaatgtagag agtgtgggaa ggccttttcc cagaagtcac agctgattat acaccacaga
     1261 gctcatactg gagagaagcc gtatgagtgt accgaatgtg ggaaagcctt ctgtgagaag
     1321 tcccacctca ttatacataa aagaattcac actggtgaga aaccctacaa atgtgctcaa
     1381 tgtgaggaag ccttcagcag gaagacagaa ctcattacac atcagttagt tcatactggg
     1441 gaaaaacctt atgaatgtac tgaatgtgga aagacattct cccgcaagtc acagctcatc
     1501 atacatcaga gaacacatac tggagaaaaa ccctataaat gtagtgaatg tggcaaagcc
     1561 ttctgccaga agtcacatct cattggacat cagagaattc acacaggaga aaaaccttat
     1621 atatgtactg aatgtgggaa agccttctct cagaagtccc accttccggg acaccagcga
     1681 attcatacag gagagaaacc ttacatatgt gctgaatgtg gaaaggcctt ttctcagaag
     1741 tcagaccttg ttttacatca gaggattcat actggggaaa gaccctatca atgtgctata
     1801 tgtgggaagg ccttcatcca gaagtcacaa ctaactgtac accagagaat tcacacagtg
     1861 gtaaaatcat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1921 atatcttgtg gaaaggacga tgaacgtttc ttgcacgtcc tccc
//
