LOCUS       sequence-281408-        1514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2831
FEATURES             Location/Qualifiers
     source          1..1514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagatat acaaacaaat gcggaactga aaagcactca ggagcagtct
       61 gtgcccgcag aaagtgcagc ggttttgaat gactacagtt taaccaaatc tcatgaaatg
      121 gaaaatgtgg acagtggaga aggcccagcc aatgaagatg aagacatagg agatgattca
      181 atgaaagtga aagatgaata cagtgaaaga gatgagaatg ttttaaagtc agaacccatg
      241 ggaaatgcag aagagcctga aatcccttac agctattcaa gagaatataa tgaatatgaa
      301 aacattaagt tggagagaca tgttgtctca ttcgatagta gcaggccaac cagtggaaag
      361 atgaactgcg atgtgtgtgg attatcctgc atcagcttca atgtcttaat ggttcataag
      421 cgaagccata ctggtgaacg cccattccag tgtaatcagt gtggggcatc ttttactcag
      481 aaaggtaacc tcctccgcca cattaaactg cacacagggg aaaaaccttt taagtgtcac
      541 ctctgcaact atgcatgcca aagaagagat gcgctcacgg ggcatcttag gacacattct
      601 gcaagtgcgg aggcaagaca catcaaagca gagatgggaa gtgaaagagc tctcgtactg
      661 gacagattag caagcaatgt ggcaaaacga aaaagctcaa tgcctcagaa attcattggt
      721 gagaagcgcc actgctttga tgtcaactat aattcaagtt acatgtatga gaaagagagt
      781 gagctcatac agacccgcat gatggaccaa gccatcaata acgccatcag ctatcttggc
      841 gccgaagccc tgcgcccctt ggtccagaca ccgcctgctc ccacctcgga gatggttcca
      901 gttatcagca gcatgtatcc catagccctc acccgggctg agatgtcaaa cggtgcccct
      961 caagagctgg aaaagaaaag catccacctt ccagagaaga gcgtgccttc tgagagaggc
     1021 ctctctccca acaatagtgg ccacgactcc acggacactg acagcaacca tgaagaacgc
     1081 cagaatcaca tctatcagca aaatcacatg gtcctgtctc gggcccgcaa tgggatgcca
     1141 cttctgaagg aggttccccg ctcttacgaa ctcctcaagc ccccgcccat ctgcccaaga
     1201 gactccgtca aagtgatcaa caaggaaggg gaggtgatgg atgtgtatcg gtgtgaccac
     1261 tgccgcgtcc tcttcctgga ctatgtgatg ttcacgattc acatgggctg ccacggcttc
     1321 cgtgaccctt tcgagtgtaa catgtgtgga tatcgaagcc atgatcggta tgagttctcg
     1381 tctcacatag ccagaggaga acacagagcc ctgctgaagt agtaatgaac tagtccgtaa
     1441 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga agatcactgt
     1501 gtcatgtcgt taac
//
