LOCUS       sequence-281420-        2024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1137
FEATURES             Location/Qualifiers
     source          1..2024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacatggg cacccaggga tcggggcgca agcggctccc caaccgggag
       61 cggctcacgg cggaggacga cgcgctcaac cagatcgcgc gggaggcgga agcccggctc
      121 gctgcaaaac gggcggcccg cgcggaggct cgcgagatcc gcatgaagga gctggagcgg
      181 cagcagaagg agatctatca ggtccaaaag aaatattatg ggctggatac aaaatggggt
      241 gacatcgagc agtggatgga agacagtgag cgctactctc gtagatccag aagaaacaca
      301 tcggcttctg atgaagacga gcgcatgtca gtgggtagtc gtggaagcct gaggtcgcag
      361 cctgacttgg agtatggggg tccttacgcc tggacaaatg gttatgatgg agaattgtat
      421 ggatcacagt ccctgaatag aagatctggc aggccctcct gtctgtacag cgctgcccgg
      481 ccttcgggga gttaccgggc gtctgtgttg gatgaaggca gcttcggtgg gacccgacgg
      541 ggcagcacct ccggctcccg tgctccctcg gagtacagcg gccacctcaa ctccagctcc
      601 cgcgcctcct ccagggccag ctcggcccgg gccagccctg tggtagaaga gagaccagaa
      661 aaagatttta ctgagaaggg gtctcgtaac atgccgggcc tgtctgcagc cacgctggcc
      721 tctctgggtg ggacttcctc tcggagaggc agcggagaca cctccatctc catcgacacc
      781 gaggcatcca tcagggaaat caaggaactc aatgagttaa aggaccagat tcaggatgta
      841 gaaggcaaat acatgcaggg attgaaagag atgaaggact ctctagcaga agttgaagag
      901 aaatataaga aggctatggt ttccaatgct cagctagaca atgaaaagac aaacttcatg
      961 taccaggttg ataccctaaa agatatgttg ctggagcttg aagaacagct ggctgaatct
     1021 aggcggcagt acgaagagaa aaacaaagaa tttgaaaggg aaaaacacgc ccacagtata
     1081 ctgcaatttc agtttgctga agtcaaggag gccctgaagc aaagagagga aatgctcgag
     1141 gaaatccgac agctacagca gaaacaggcg agttctatca gggagatttc tgatcttcag
     1201 gaaacaatag agtggaaaga caaaaagata ggggcattag agaggcagaa agagttcttt
     1261 gattccgtaa ggagtgaacg ggatgatctt agagaagaag tagtcatgct gaaagaggaa
     1321 ttaaagaaac atggaataat cctaaattca gaaatagcta ccaatggaga gacttccgac
     1381 accctcaata atgttggata ccaaggtcct accaagatga caaaagaaga gttaaatgcc
     1441 ctcaagtcga caggggatgg gaccctagat attaggttga aaaagctggt tgatgaacgg
     1501 gaatgcttat tggaacagat taagaaactc aaagggcagc tggaggagag acagaagatt
     1561 ggcaaactag acaatcttcg atctgaagat gatgtcttgg aaaacgggac agacatgcat
     1621 gtaatggacc tacaaaggga tgccaacaga cagatcagcg acctcaaatt taaacttgca
     1681 aaatctgagc aagagataac tgcattagaa caaaatgtaa taaggttaga gagtcaagta
     1741 tcacgttaca aatcagcggc tgaaaatgca gaaaaaatag aagatgaact taaggcagaa
     1801 aaacggaaac tccaaagaga gctccgctct gcattggata aaacagaaga gctcgaggtg
     1861 agcaacggcc acttagtgaa gcgtctggaa aaaatgaaag caaatcggag tgcactcttg
     1921 tcccagcagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1981 atatcttgtg gaaaggacga gacacacttg ccgtgtagtg atac
//
