LOCUS       sequence-281429-        1601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1276
FEATURES             Location/Qualifiers
     source          1..1601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gcaatttaga gatgtggcca tagaattctc tctggaggag
       61 tggcattgcc tggacactgc acagcggaat ttatataggg atgtgatgtt agagaactac
      121 agaaacttgg tcttccttgg tattgttgtc tctaagccag acctggttac ctgtctggag
      181 caaggaaaaa aacctttaac tatggaaaga catgagatga ttgccaaacc cccagttatg
      241 agttctcatt ttgcccaaga cctttggcca gagaacatac aaaattcttt ccaaataggg
      301 atgctgagaa gatatgaaga atgcagacat gacaatttac agttaaaaaa aggctgtaaa
      361 agcgtgggtg agcataaggt gcacaaagga ggttataatg gacttaacca atgtttgaca
      421 actacccaga aagaaatatt tcaatgtgat aaatatggaa aagtctttca taagttttca
      481 aattcaaaca catataagac aagacatact ggaataaatc ttttcaaatg tataatatgt
      541 ggcaaagctt ttaaacggtc ctcaaccctt actacacata agaaaattca tactggagag
      601 aaaccttaca gatgtgaaga atgtggcaaa gcttttaacc aatctgcaaa ccttactaca
      661 cataagagaa ttcataccgg agagaaaccc tacagatgtg aagaatgtgg caaagccttt
      721 aagcagtcct caaaccttac tacacataag aaaattcata ctggagagaa accctacaaa
      781 tgtgaagaat gtggcaaagc cttcaaccga tccacagacc ttactacaca taagatagtt
      841 catactggag agaaacccta caaatgtgaa gaatgtggca aagcctttaa gcacccctca
      901 cacgttacca cacataagaa aattcatact agagggaaac cctacaactg tgaagaatgt
      961 ggcaaatcct ttaagcactg ctctaacctt actatacata agagaattca tacaggagag
     1021 aaaccctaca aatgtgaaga atgtggcaaa gcctttcacc tatcctcaca ccttactaca
     1081 cataagatac ttcatactgg agagaaaccc tacagatgta gagaatgtgg caaagctttt
     1141 aaccattcca caaccctttt ttcacatgag aaaattcata ctggagagaa accctacaaa
     1201 tgtgatgaat gtggcaaaac ctttacctgg ccctcaatcc tctccaaaca taaaagaact
     1261 catactggag agaaacccta caaatgtgaa gaatgtggca aatcctttac tgcatcctca
     1321 actctaacta cacataagag aattcatact ggagagaaac cttacaaatg tgaagaatgt
     1381 ggcaaagctt ttaactggtc ctcagacctt aataaacata agaaaattca tattgaacga
     1441 aaaccctaca tagtgaagaa tgtgacagat cttttaaatg ttcctccact tttaattagc
     1501 ataagatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1561 tcttgtggaa aggacgattg gtgaacctac aatgacaagg g
//
