LOCUS       sequence-281435-        4142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1346
FEATURES             Location/Qualifiers
     source          1..4142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccagta cagccttgga gacgagggtg ccctcccatc agaaatgcac
       61 ctcccttcat tttcagagag ccaagggctc aactgcagcg acaccctcaa ccgggatttg
      121 ggtcccaaca cgcgaggctt tctttatgct ggcctgagtg gtctggaccc ggaccccagc
      181 ctcccaacgc ctgacatgtc cagcgaggtg ctggaggaca acttagacac cttgtccctg
      241 tactccggga aggacagtga ttctgtgaag ctgctggagg agtacgcaga ttcggagtct
      301 caggcatcct tacaagacct ggggctaggt gtgcttaagg ctaaagaggc tgacgaagga
      361 ggaagggcca cctcggggag tgccaggaaa ggaaagcggc agcacagttc ccctcaaaac
      421 ccacttctgg actgcagcct gtgcgggaag gtgttcagca gcgccagttc tctgagcaag
      481 cactacctga cacacagcca ggaaaggaag cacgtctgca aaatctgcag caaggccttt
      541 aagcgccagg accacctgac cgggcacatg ctcacccacc agaagacaaa gcccttcgtg
      601 tgcatcgagc agggctgcag caagagctac tgcgactacc gctctctgcg ccggcactac
      661 gaggtccatc acggcctgtg catcctgaag gaagcccccc cggaggaaga ggcctgcggg
      721 gactcccccc acgcccacga gtcggccggc cagccgcccc ccagcagcct gcggtccctg
      781 gtgcccccag aggccaggtc ccccggctcc ctcctgcccc accgggacct cctgcgccgc
      841 atcgtgagta gcatcgtcca ccagaagacc ccttctcctg gcccagcccc ggcgggggct
      901 tcagacagcg aagggaggaa cactgcctgt ccctgccccg cctcatcggg gtcctcgtcc
      961 tgcaccccag ccggccccca cgcggcccca gcagcgctgg acaccgagct tcccgaggag
     1021 ccttgcctcc cacagaaaga gccggccact gacgtgttca cagcccctaa ttccagggcc
     1081 gccgagaacg gcgcccccga cccgccagag ccggagccag ataccgcgct gctccaggcc
     1141 cggtccaccg cggagtgctg gcccgaaggc ggctccgtgc ctgcctgcct gcctctcttc
     1201 cgaggccaga cggtccctgc cagttcccag ccatcgagcc acagcttcca gtggctccgg
     1261 aacctgccgg gctgtcccaa aagcaaaggc aacaacgtgt ttgttgtcca caagccctcg
     1321 gccgtgccct cgcgggaggg ctccgagtct ggcccgggac ccagcagcgg aagcccctcg
     1381 gaggagtccc cgcccggccc cggcggtggc ctggaggatg ctctgccctt ccctgccgcg
     1441 ctcctcaggg tccccgcgga ggccccgagc gaccccaggt cggccagcgg ggaagatgac
     1501 ccctgcgccc ccaagaaggt caaggtcgac tgcgactcct tcctgtgcca gaaccccggg
     1561 gagcccggcc tccaggaggc ccagaaggca ggcgggctcc ctgcggatgc ctcgccgctc
     1621 ttccgccagc tcttcctcaa gtcgcaggaa cctcttgtga gccacgagca gatgcaggtg
     1681 ttccagatga tcaccaagtc ccagcggatc ttctcccatg cccaggtggc agcagtctcc
     1741 tcccagctcc ctgcgcccga gggcaaacca gccgccctga ggccgctgca ggggccgtgg
     1801 ccgcagcagc ccccaccact ggctcctgct gtggactctc tccacgccgg ccctggaaac
     1861 cccgaggcag agggctcccc agcccgcagg agaaaaacca cacccggggt tcccagagag
     1921 gcctcccccg gcagcacgag acgagacgca aaggggggac tgaaagtggc cgcggttcca
     1981 acgccccttg cagcaccgtc tctggaccct tccaggaatc cagacatctc ttctctggcc
     2041 aagcagctgc gatcctctaa agggaccttg gacctggagg acatcttccc ctccacaggc
     2101 caacggcaga cccagttagg tggggaggag ccaccaggag cctcgctgcc agggaagcag
     2161 gccccagccg agaatggcgc ggcttcaagg atcacaaaag gtgaaaaggg cccagcctgc
     2221 tcccggggtg gaggctaccg gctcttgggc aaccccaggg ccccgcgatt ctccggcttc
     2281 cggaaagaga aggcgaagat ggatatgtgc tgtgcggctt ctccgagcca ggtagccatg
     2341 gcctccttct catcggccgg gcccccggca gatccctcca agtccaagct gacaatattc
     2401 agcagaatcc agggtggaaa catctacagg ctcccccatc cggtgaagga agagaatgtg
     2461 gcaggcagag gtaaccagca aaacggcagt cccacagact ggacgaagcc caggagcact
     2521 tttgtctgca agaactgcag ccagatgttt tatacggaga aagggctgag cagccacatg
     2581 tgttttcaca gcgaccagtg gccgtcacct cgagggaagc aggaaccgca ggtgtttggc
     2641 acagagtttt gcaagccgct aagacaggtg ctgaggccag aaggggacag gcatagtccc
     2701 ccaggaacca agaagccctt ggaccccaca gctgcagccc ctttggtggt cccccaatcg
     2761 atccccgtgg ttccagtgac ccgacacata gggagcatgg ccatgggaca agagaaagac
     2821 ggggaggagc gagacagcaa ggagagcagc cagcagagaa agcggaagaa gcggccccca
     2881 ccctccacgg ctggggagcc tggccctgca ggatgccacc agagccgcct gcggtcaccc
     2941 atgttcctgg tggactgcct cctgaagggc ttattccagt gctcccccta cacaccaccc
     3001 ccaatgctca gccccatccg ggagggctct ggggtgtact tcaacaccct ctgttccacg
     3061 tccactcagg ccagccctga ccagctcatc agctccatgc tcgatcaagt ggatgggtcc
     3121 tttggcatct gtgtggtgaa ggatgacacc aaaatcagca ttgagccaca tatcaatata
     3181 ggaagccggt ttcaggcaga gatcccggaa ctccaggaga gatccctggc tggaactgac
     3241 gagcatgtag cttctctggt ctggaagcca tggggagata tgatgatcag ctcagagaca
     3301 caggatagag tgaccgagct ctgcaatgtg gcatgctcca gcgtgatgcc aggagggggc
     3361 accaacctgg agctcgctct gcactgcctg cacgaggctc agggcaacgt tcaggtcgcc
     3421 ctggagactc ttctgctccg agggccccac aagccacgga cacacctgct cgctgactat
     3481 cgctacacag gttcagacgt ctggacccct atagagaaga ggctttttaa gaaggcgttc
     3541 tatgcccaca agaaggactt ctacttgata cacaagatga tccagacaaa gacggtagct
     3601 cagtgcgttg agtattatta catctggaaa aaaatgatca agtttgactg tggccgagcc
     3661 ccagggctag aaaagagggt caagagagag ccggaggaag tggaaaggac agaggaaaag
     3721 gtcccatgca gccctcggga gagacccagc caccatccaa ctcccaagtt aaagaccaag
     3781 agttatagga gggagtccat cctcagctcc agcccaaatg caggctccaa gcggaccccc
     3841 gagctgttgg gaagtgcaga gagccagggc atttttccat gcagagagtg tgaaagggtg
     3901 tttgacaaga tcaagagtcg aaatgcccat atgaagcggc accgccttca ggaccacgtg
     3961 gagcccatca tcagggtgaa gtggccagtg aagcccttcc agctaaagga agaggagctg
     4021 ggagctgaca tcggccccct gcagtggtag taatgaacta gtccgtaact tgaaagtatt
     4081 tcgatttctt ggctttatat atcttgtgga aaggacgatg cttaatatgc cgagcctcgt
     4141 at
//
