LOCUS       sequence-281444-        1460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1360
FEATURES             Location/Qualifiers
     source          1..1460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaggtgg gggcggcgac ctgagcacca ggatgttcac ggaagaccag
       61 ggtgtagatg acaggctgct ctatgacatt gtattcaagc acttcaaaag aaataaggtg
      121 gagatttcaa atgcaataaa aaagacattt ccattcctcg agggcctccg tgatcgtgat
      181 ctcatcacaa ataaaatgtt tgaagattct caagattctt gtagaaacct ggtccctgta
      241 cagagagtgg tgtacaatgt tcttagtgaa ctggagaaga catttaacct gccagttctg
      301 gaagcactgt tcagcgatgt caacatgcag gaataccccg atttaattca catttataaa
      361 ggctttgaaa atgtaatcca tgacaaattg cctctccaag aaagtgaaga agaagagagg
      421 gaggagaggt ctggcctcca actaagtctt gaacaaggaa ctggtgaaaa ctcttttcga
      481 agcctgactt ggccaccttc gggttcccca tctcatgctg gtacaacccc acctgaaaat
      541 ggactctcag agcacccctg tgaaacagaa cagataaatg caaagagaaa agatacaacc
      601 agtgacaaag atgattcgct aggaagccaa caaacaaatg aacaatgtgc tcaaaaggct
      661 gagccaacag agtcctgcga acaaattgct gtccaagtga ataatgggga tgctggaagg
      721 gagatgccct gcccgttgcc ctgtgatgaa gaaagcccag aggcagagct acacaaccat
      781 ggaatccaaa ttaattcctg ttctgtgcga ctggtggata taaaaaagga aaagccattt
      841 tctaattcaa aagttgagtg ccaagcccaa gcaagaactc atcataacca ggcatctgac
      901 ataatagtca tcagcagtga ggactctgaa ggatccactg acgttgatga gcccttagaa
      961 gtcttcatct cagcaccgag aagtgagcct gtgatcaata atgacaaccc tttagaatca
     1021 aatgatgaaa aggagggcca agaagccact tgctcacgac cccagattgt accagagccc
     1081 atggatttca gaaaattatc tacattcaga gaaagtttta agaaaagagt gataggacaa
     1141 gaccacgact tttcagaatc cagtgaggag gaggcgcccg cagaagcctc gagcggggca
     1201 ctgagaagca agcatgctcc tatgacttct agaagtacat ctacttggag aatacccagc
     1261 aggaagagac gtttcagcag tagtgacttt tcagacctga gtaatggaga agagcttcag
     1321 gaaacctgca gctcatccct aagaagaggg tcaggtaaag aagattagta atgaactagt
     1381 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgataca
     1441 tcaaaccatc acctgtttcc
//
