LOCUS       sequence-281447-        1181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1366
FEATURES             Location/Qualifiers
     source          1..1181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtggatat tatttttcat tatccttttc tgggtgctat gggtgatcat
       61 tccaagaaga agcccgggac ggccatgtgc gtgggctgcg ggagtcagat ccacgaccag
      121 tttatcctgc gggtgtcgcc cgacctcgag tggcacgcgg cctgcctcaa gtgtgccgag
      181 tgcagccagt acctggacga gacgtgcacg tgcttcgtga gagacgggaa gacctactgc
      241 aagcgggact atgtcaggct gttcggcatc aagtgcgcca agtgccaggt gggcttcagc
      301 agcagcgacc tggtgatgag ggcgcgggac agcgtgtacc acatcgagtg cttccgctgc
      361 tccgtgtgca gccgccagct gctgcctggg gacgagttct cgctgcggga gcacgagctg
      421 ctctgccgcg ccgaccacgg cctcctgctc gagcgcgccg cggccggcag cccgcgcagc
      481 cccggcccgc ttcccggcgc ccgcggcctg catctgcccg acgctgggtc gggccggcag
      541 cccgcgttgc gcccgcacgt gcacaagcag acggagaaga cgacccgcgt gcggactgtg
      601 ctgaacgaga agcagctgca cactctgcgg acctgctacg ccgccaaccc gcggcccgac
      661 gctctcatga aggagcagct ggtggagatg accggcctga gcccgcgggt catccgcgtc
      721 tggttccaga acaagcgctg caaggacaag aagaaatcca ttctcatgaa gcagctgcag
      781 cagcagcagc acagcgacaa gacgagcctt cagggactga ctgggacgcc cctggtggcg
      841 ggcagtccca tccgccatga gaacgccgtg cagggcagcg cagtggaggt gcagacgtac
      901 cagccgccgt ggaaggcgct cagcgagttt gccctccaga gcgacctgga ccaacccgcc
      961 ttccaacagc tggtctcctt ctccgagtcc ggctccctag gcaactcctc cggcagcgac
     1021 gtgacctccc tgtcctcgca gctcccggac acccccaaca gtatggtgcc gagtcccgtg
     1081 gagacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1141 tcttgtggaa aggacgagac cgctcggacc cgctacggta g
//
