LOCUS       sequence-281462-        1361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1404
FEATURES             Location/Qualifiers
     source          1..1361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcagga gtcagtgatg ttcagtgatg tgtccgtaga cttctctcag
       61 gaggagtggg aatgcctgaa tgatgatcag agagatttat acagagatgt gatgttggag
      121 aattacagca acctggtttc aatggcaggg cattctattt ctaaaccaaa tgtgatctcc
      181 tacttggagc aagggaagga gccctggttg gctgacagag agctaacaag aggccagtgg
      241 ccagtcctgg aatcaagatg tgagaccaag aaattatttc tgaagaaaga aatttatgaa
      301 atagaatcaa cccagtggga aataatggaa aaactcacaa gacgtgattt tcagtgctcc
      361 agtttcagag atgattggga atgtaatcgg cagtttaaga aagaactcgg ctctcagggg
      421 ggacatttca atcaattggt attcactcat gaagatctgc ccactttgag tcaccatcca
      481 tccttcacat tacagcaaat cattaacagt aaaaagaaat tctgtgcatc taaagaatat
      541 aggaaaacct ttagacatgg ctcacagttt gctacacatg agataattca taccattgag
      601 aagccttatg aatgtaagga atgtggaaag tcctttagac atccctcaag actcactcat
      661 catcagaaaa ttcatactgg caagaaaccc tttgaatgta aggaatgtgg aaaaaccttt
      721 atttgtggct cagaccttac tcgacatcac agaattcaca ctggtgagaa accctatgaa
      781 tgtaaggaat gtgggaaagc ctttagtagt ggttcaaact tcactcgaca tcagagaatt
      841 cacacaggtg agaagcctta tgaatgcaaa gaatgcggga aggcctttag tagtggctca
      901 aactttactc aacatcagag aattcatact ggggaaaaac cctatgaatg taaggaatgt
      961 ggcaatgcct ttagtcagag ctcacaactt attaaacatc aaagaatcca tacaggtgag
     1021 aaaccttacg aatgtaagga atgtgaaaag gcttttcgtt ctggctcaga ccttactaga
     1081 catcagagaa ttcatactgg ggagaaaccc tatgaatgta agatatgtgg gaaggcttat
     1141 tctcagagtt cacagcttat tagtcatcat agaattcata ctagtgagaa accctatgaa
     1201 tatagggaat gtggaaagaa ctttaattat gacccacaac ttattcagca tcaaaatttg
     1261 tactggtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1321 tcttgtggaa aggacgaaca acggtgtgaa gacacgtgag c
//
