LOCUS       sequence-281465-        1358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1405
FEATURES             Location/Qualifiers
     source          1..1358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcagga gtcagtgatg ttcagtgatg tgtccgtaga cttctctcag
       61 gaggagtggg aatgcctgaa tgatgatcag agagatttat acagagatgt gatgttggag
      121 aattacagca acctggtttc aatggggcat tctatttcta aaccaaatgt gatctcctac
      181 ttggagcaag ggaaggagcc ctggttggct gacagagagc taacaagagg ccagtggcca
      241 gtcctggaat caagatgtga gaccaagaaa ttatttctga agaaagaaat ttatgaaata
      301 gaatcaaccc agtgggaaat aatggaaaaa ctcacaagac gtgattttca gtgctccagt
      361 ttcagagatg attgggaatg taatcggcag tttaagaaag aactcggctc tcagggggga
      421 catttcaatc aattggtatt cactcatgaa gatctgccca ctttgagtca ccatccatcc
      481 ttcacattac agcaaatcat taacagtaaa aagaaattct gtgcatctaa agaatatagg
      541 aaaaccttta gacatggctc acagtttgct acacatgaga taattcatac cattgagaag
      601 ccttatgaat gtaaggaatg tggaaagtcc tttagacatc cctcaagact cactcatcat
      661 cagaaaattc atactggcaa gaaacccttt gaatgtaagg aatgtggaaa aacctttatt
      721 tgtggctcag accttactcg acatcacaga attcacactg gtgagaaacc ctatgaatgt
      781 aaggaatgtg ggaaagcctt tagtagtggt tcaaacttca ctcgacatca gagaattcac
      841 acaggtgaga agccttatga atgcaaagaa tgcgggaagg cctttagtag tggctcaaac
      901 tttactcaac atcagagaat tcatactggg gaaaaaccct atgaatgtaa ggaatgtggc
      961 aatgccttta gtcagagctc acaacttatt aaacatcaaa gaatccatac aggtgagaaa
     1021 ccttacgaat gtaaggaatg tgaaaaggct tttcgttctg gctcagacct tactagacat
     1081 cagagaattc atactgggga gaaaccctat gaatgtaaga tatgtgggaa ggcttattct
     1141 cagagttcac agcttattag tcatcataga attcatacta gtgagaaacc ctatgaatat
     1201 agggaatgtg gaaagaactt taattatgac ccacaactta ttcagcatca aaatttgtac
     1261 tggtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1321 tgtggaaagg acgagggtgc cgtgggaaga tgaaacga
//
