LOCUS       sequence-281480-        2705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1428
FEATURES             Location/Qualifiers
     source          1..2705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaccaa aggctaccac agtctccctg aaggtctaga tatggaaaga
       61 cggtggggtc aagtttctca ggctgtggag cgttcttccc tgggacctac agagaggacc
      121 gatgagaata actacatgga gattgtcaac gtaagctgtg tttccggtgc tattccaaac
      181 aacagtactc aaggaagcag caaagaaaaa caagaactac tcccttgcct tcagcaagac
      241 aataatcggc ctgggatttt aacatctgat attaaaactg agctggaatc taaggaactt
      301 tcagcaactg tagctgagtc catgggttta tatatggatt ctgtaagaga tgctgactat
      361 tcctatgagc agcagaacca acaaggaagc atgagtccag ctaagattta tcagaatgtt
      421 gaacagctgg tgaaatttta caaaggaaat ggccatcgtc cttccactct aagttgtgtg
      481 aacacgccct tgagatcatt tatgtctgac tctgggagct ccgtgaatgg tggcgtcatg
      541 cgcgccgttg ttaaaagccc tatcatgtgt catgagaaaa gcccgtctgt ttgcagccct
      601 ctgaacatga catcttcggt ttgcagccct gctggaatca actctgtgtc ctccaccaca
      661 gccagctttg gcagttttcc agtgcacagc ccaatcaccc agggaactcc tctgacatgc
      721 tcccctaatg ttgaaaatcg aggctccagg tcgcacagcc ctgcacatgc tagcaatgtg
      781 ggctctcctc tctcaagtcc gttaagtagc atgaaatcct caatttccag ccctccaagt
      841 cactgcagtg taaaatctcc agtctccagt cccaataatg tcactctgag atcctctgtg
      901 tctagccctg caaatattaa caactcaagg tgctctgttt ccagcccttc gaacactaat
      961 aacagatcca cgctttccag tccggcagcc agtactgtgg gatctatctg tagccctgta
     1021 aacaatgcct tcagctacac tgcttctggc acctctgctg gatccagtac attgcgggat
     1081 gtggttccca gtccagacac gcaggagaaa ggtgctcaag aggtcccttt tcctaagact
     1141 gaggaagtag agagtgccat ctcaaatggt gtgactggcc agcttaatat tgtccagtac
     1201 ataaaaccag aaccagatgg agcttttagc agctcatgtc taggaggaaa tagcaaaata
     1261 aattcggatt cttcattctc agtaccaata aagcaagaat caaccaagca ttcatgttca
     1321 ggcacctctt ttaaagggaa tccaacagta aacccgtttc catttatgga tggctcgtat
     1381 ttttccttta tggatgataa agactattat tccctatcag gaattttagg accacctgtg
     1441 cccggctttg atggtaactg tgaaggcagc ggattcccag tgggtattaa acaagaacca
     1501 gatgatggga gctattaccc agaggccagc atcccttcct ctgctattgt tggggtgaat
     1561 tcaggtggac agtccttcca ctacaggatt ggtgctcaag gtacaatatc tttatcacga
     1621 tcggctagag accaatcttt ccaacacctg agttcctttc ctcctgtcaa tactttagtg
     1681 gagtcatgga aatcacacgg cgacctgtcg tctagaagaa gtgatgggta tccggtctta
     1741 gaatacattc cagaaaatgt atcaagctct actttacgaa gtgtttctac tggatcttca
     1801 agaccttcaa aaatatgttt ggtgtgtggg gatgaggctt caggatgcca ttatggggta
     1861 gtcacctgtg gcagctgcaa agttttcttc aaaagagcag tggaagggca acacaactat
     1921 ttatgtgctg gaagaaatga ttgcatcatt gataagattc gacgaaagaa ttgtcctgct
     1981 tgcagacttc agaaatgtct tcaagctgga atgaatttag gaggatttaa aaacttgcct
     2041 cttgaggacc aaattaccct aatccagtat tcttggatgt gtctatcatc atttgccttg
     2101 agctggagat cgtacaaaca tacgaacagc caatttctct attttgcacc agacctagtc
     2161 tttaatgaag agaagatgca tcagtctgcc atgtatgaac tatgccaggg gatgcaccaa
     2221 atcagccttc agttcgttcg actgcagctc acctttgaag aatacaccat catgaaagtt
     2281 ttgctgctac taagcacaat tccaaaggat ggcctcaaaa gccaggctgc atttgaagaa
     2341 atgaggacaa attacatcaa agaactgagg aagatggtaa ctaagtgtcc caacaattct
     2401 gggcagagct ggcagaggtt ctaccaactg accaagctgc tggactccat gcatgacctg
     2461 gtgagcgacc tgctggaatt ctgcttctac accttccgag agtcccatgc gctgaaggta
     2521 gagttccccg caatgctggt ggagatcatc agcgaccagc tgcccaaggt ggagtcgggg
     2581 aacgccaagc cgctctactt ccaccggaag tagtaatgaa ctagtccgta acttgaaagt
     2641 atttcgattt cttggcttta tatatcttgt ggaaaggacg aagagggagt atgttatcct
     2701 cgcgt
//
