LOCUS       sequence-281486-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1430
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacctgca gaatgaccta ggccagacag ccctgcacct ggcagccatc
       61 ctgggggaga catccacggt ggagaagctg tacgcagcag gcgccgggct gtgtgtggcg
      121 gagcgtaggg gccacacggc gctgcacctg gcctgccgtg tgggggcaca cgcctgtgcc
      181 cgtgccctgc ttcagccccg cccccggcgc cccagggaag cccccgacac ctacctcgct
      241 cagggccctg accgtactcc cgacaccaac catacccctg tcgccttgta ccccgattcc
      301 gacttggaga aggaagaaga ggagagtgag gaggactgga agctgcagct ggaggctgaa
      361 aactacgagg gccacacccc actccacgtg gccgttatcc acaaagatgt ggagatggtc
      421 cggctgctcc gagatgctgg agctgacctt gacaaaccgg agcccacgtg cggccggagc
      481 ccccttcatt tggcagtgga ggcccaggca gccgatgtgc tggagcttct cctgagggca
      541 ggcgcgaacc ctgctgcccg catgtacggt ggccgcaccc cactcggcag tgccatgctc
      601 cggcccaacc ccatcctcgc ccgcctcctc cgtgcacacg gagcccctga gcccgagggc
      661 gaggacgaga aatccggccc ctgcagcagc agtagcgaca gcgacagcgg agacgagggc
      721 gatgaatacg acgacattgt ggttcacagc agccgcagcc aaacccggct gcctcccacc
      781 ccagcctcaa aacctcttcc tgacgacccc cgccccgtgt agtaatgaac tagtccgtaa
      841 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tgttaccact
      901 gtcgagggta acgc
//
