LOCUS       sequence-281489-        1703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1439
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggattcttc tcagcactta gtgacctttg aggatgtggc tgtggacttt
       61 acccaggagg agtggacttt gttggatcaa gcccagagag atctctacag agatgtgatg
      121 ttggagaact acaagaatct cattatacta gcagggtctg aattattcaa acgtagtctc
      181 atgtctggat tggaacaaat ggaagagctg aggacaggag tgacaggagt tctgcaggaa
      241 ttggatttgc aactcaaaac caaaggctcc ccactgctgc aagatatttc tgcagaaaga
      301 tcaccaaatg gagtacaatt ggagagaagc aatactgcag agaaactgta tgactctaac
      361 cattctggaa aagtcttcaa tgaacaccca tttcttatga ctcacatgat aactcacatt
      421 ggagagaaaa cttctgagga taatcagagt ggaaaagcct taagaaagaa ctttcctcat
      481 agtttttaca agaaaagtca tgctgagggg aaaatgccta agtgtgttaa acatgaaaaa
      541 gccttcaacc agtttccaaa tcttactagg cagaataaaa ctcacacaca agagaaattg
      601 tgtgaatgca aagactgttg gagaactttt cttaatcagt catcccttaa gttacatata
      661 agatctcaca atggagacaa acactatgta tgtaaggaat gtgggaaagc cttcagtaat
      721 tcctcacacc ttataggaca tggaagaatt cacagtggag agaagcccta tgtctgtaaa
      781 gaatgtggta aagctttcac tcaatccaca ggacttaaat tacacatcag aactcacagt
      841 ggagaaaaac catataaatg taaagagtgt gggaaagcct tcacccattc ttcatacctt
      901 actgatcata caagaatcca cagtggaaag aagccctatg tatgtatgga atgtggaaaa
      961 gccttcacta gatccacagg acttatttta cacatgcgaa ttcacactgg agaaaagcca
     1021 tatgaatgta aggagtgtgg aaaagctttt attcattcct cataccttac aaaacatgta
     1081 aggattcaca gtggagagaa gctgtattta tgtaaggcat gtgggaaagc ttttactcgt
     1141 tcctcaggac ttgttttaca catgagaaca catactggag aaaagcccta tgaatgtaaa
     1201 gaatgtggga aagcctttaa taattcctca atgcttagtc aacatgtaag gattcacact
     1261 ggagagaagc catatgaatg caaagaatgt gggaaagctt tcactcaatc ctcgggcctt
     1321 agtacccatt taagaactca cactggagaa aaggcctgtg aatgtaagga atgcggtaaa
     1381 gcatttgctc gttccacaaa tcttaatatg cacatgcgaa cgcacacagg agaaaagcct
     1441 tatgcatgta aagaatgtgg gaaagccttc aggtattcca cataccttaa cgttcacaca
     1501 cgaactcaca ctggagcaaa accatatgaa tgtaagaaat gtgggaaaaa cttcactcaa
     1561 tcttcagcac ttgctaaaca tctaagaact aaggcatgtg aaaaaaccta gtaatgaact
     1621 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1681 ccctccggaa agggactgtg tgt
//
