LOCUS       sequence-281513-        1574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1495
FEATURES             Location/Qualifiers
     source          1..1574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtccagt catgcctccc agtaagaagc cagaaagctc aggaattagt
       61 gtctccagtg gactgagtca gtgttacggg ggcagcggtt tctccaaggc ccttcaggaa
      121 gacgatgacc tcgacttttc tctgcctgac atccgattag aagagggggc catggaagat
      181 gaagagctga ccaacctgaa ctggctgcac gagagcaaga acttgctgaa gagctttggg
      241 gagtcggtcc tcaggagtgt cagccccgtc caggacctgg acgatgacac ccccccatcc
      301 cctgcccact ctgacatgcc ctacgatgcc aggcagaacc ccaactgcaa acccccctac
      361 tccttcagct gcctcatatt tatggccatc gaggactctc caaccaagcg cctgccagtg
      421 aaggatatct acaactggat cttggaacat tttccgtatt ttgcaaatgc acctactggg
      481 tggaaaaact cagtgagaca caatttatca ttgaataagt gttttaagaa agtggacaaa
      541 gagaggagtc agagtattgg gaaagggtcg ttgtggtgca tagacccaga gtatagacaa
      601 aatctaattc aggctttgaa aaagacacct tatcacccac acccacacgt gttcaataca
      661 cctcccacct gtcctcaggc atatcaaagc acatcaggtc cacccatctg gccgggcagt
      721 accttcttca agagaaatgg agcccttctc caagatcctg acattgatgc tgccagtgcc
      781 atgatgcttt tgaatactcc ccctgagata caagcaggtt ttcctccagg agtgatccaa
      841 aatggagcgc gggtcctgag ccgagggctg tttcctggcg tgcggccgct gccaatcact
      901 cccattgggg tgacagcggc catgaggaat ggcatcacca gctgccggat gcggactgag
      961 agtgagccat cttgtggctc cccagtggtc agcggagacc ccaaggagga tcacaactac
     1021 agcagtgcca agtcctccaa cgcccggagc acctcgccca ccagcgactc catctcctcc
     1081 tcctcctcct cagccgacga ccactatgag tttgccacca aggggagcca ggagggcagc
     1141 gagggcagcg aggggagctt ccggagccac gagagcccca gcgacacgga agaggacgac
     1201 aggaagcaca gccagaagga gcccaaggat tctctggggg acagcgggta cgcatcccag
     1261 cacaagaagc gccagcactt cgccaaggcc aggaaggtcc ccagcgacac actgcccctc
     1321 aaaaagagac gcaccgaaaa gccccccgag agcgatgatg aggagatgaa agaagcggca
     1381 gggtccctcc tgcacttagc agggatccgg tcctgtttga ataacatcac caatcggacg
     1441 gcaaaggggc agaaagagca aaaggaaacc acaaaaaatt agtaatgaac tagtccgtaa
     1501 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tgttctgtct
     1561 cacctacaca ccga
//
