LOCUS       sequence-281525-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1528
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcaaacg cgggcggccg cgcaaggagg cgcgctgcga gggcgcgggg
       61 ctggcccccg ccgcgccccc ggctgtgccc cccgccgtgg ccgcgcccca gcccccggcc
      121 ctgcccgagg accccgctgg ggccaagccc aggtgcccct tctcagacat tttcaacacc
      181 agcgagaact cgatggagaa gcacatcaac acttttctgc agaacgtgca gattctgctc
      241 gaggccgcca gctacctgga gcagatcgag aaagaaaaca aaaagtgtga acatggctac
      301 gcctcttcat tcccgtccat gccgagcccc cgactgcagc attcaaagcc cccacggagg
      361 ttgagccggg cacagaaaca cagcagcggg agcagcaaca ccagcactgc caacagatct
      421 acacacaatg agctggaaaa gaatcgacga gctcatctgc gcctttgttt agaacgctta
      481 aaagttctga ttccactagg accagactgc acccggcaca caacacttgg tttgctcaac
      541 aaagccaaag cacacatcaa gaaacttgaa gaagctgaaa gaaaaagcca gcaccagctc
      601 gagaatttgg aacgagaaca gagattttta aagtggcgac tggaacagct gcagggtcct
      661 caggagatgg aacgaatacg aatggacagc attggatcaa ctatttcttc agatcgttct
      721 gattcagagc gagaggagat tgaagtggat gttgaaagca cagagttctc ccatggagaa
      781 gtggacaata taagtaccac cagcatcagt gacattgatg accacagcag cctgccgagt
      841 attgggagtg acgagggtta ctccagtgcc agtgtcaaac tttcattcac ttcatagtaa
      901 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      961 gacgagatcg gtactgggct gcactaaag
//
