LOCUS       sequence-281528-        2399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1540
FEATURES             Location/Qualifiers
     source          1..2399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccctgt tgctacccac agctgccatc tgctccagca actgcatgag
       61 cagcgaatcc aaggcctgct ttgtgactgt atgttggtgg taaaaggagt ctgctttaaa
      121 gcgcataaga atgtcctggc agcattcagc cagtatttta ggagcctctt tcagaattct
      181 tcaagccaga agaatgatgt ttttcacttg gatgttaaaa atgtcagtgg catagggcag
      241 atcctggact tcatgtacac ttctcatcta gatcttaacc aggacaatat acaagtaatg
      301 ctggacacag cacagtgttt gcaagttcaa aatgttctga gtctgtgtca cacattttta
      361 aaatcagcca ctgtagtaca gccacctggc atgccttgta atagtacatt gtctctacaa
      421 agcaccctga ccccagatgc cacttgtgtt atcagtgaaa actacccccc tcatttactg
      481 caggaatgtt cagcagatgc acagcagaac aaaacgttgg atgaatcgca tccgcatgct
      541 tcaccatcag ttaatcgtca tcactccgca ggtgaaatct caaaacaagc tcctgatact
      601 tcagatggca gctgcacaga actgcctttc aaacagccaa attactatta caaactcaga
      661 aacttttaca gtaagcagta ccataaacac gcagctggtc ccagtcagga gagagttgtt
      721 gagcagcctt ttgctttcag cacctctaca gaccttacca cggtagagag ccagccttgt
      781 gccgtcagtc attctgaatg catcctggag tctcccgagc acttaccttc caacttcctg
      841 gcccagcctg tgaatgactc tgccccacac cctgagtcag acgccacatg ccaacaacct
      901 gtcaagcaga tgaggctcaa aaaggccatt catctgaaga agctcaattt cctgaagtca
      961 cagaaatacg cagagcaagt atctgaaccc aagtcagatg atggtttgac aaagaggttg
     1021 gaatctgcta gtaaaaatac cctagagaaa gctagcagcc aaagtgctga agaaaaagaa
     1081 agtgaagaag tcgtcagttg tgagaatttt aattgcatta gtgagacgga gaggcctgaa
     1141 gacccggctg ccctggaaga ccagtcccag acacttcagt cccagagaca atacgcgtgt
     1201 gaattatgcg ggaaaccttt taaacaccca agcaacttgg agcttcacaa acggtctcat
     1261 acaggtgaga aaccttttga atgtaacatt tgtgggaaac atttctctca ggcaggtaac
     1321 ttgcagactc acttacgacg gcattctggt gaaaaaccat acatctgcga gatctgtgga
     1381 aagaggtttg cagcctctgg cgacgtccag cgtcacatta ttattcactc aggagaaaaa
     1441 ccacacttgt gtgacatctg tggtcgaggg tttagtaact tcagtaattt gaaggagcac
     1501 aaaaagacac acacggctga taaagtcttc acctgtgatg agtgtggaaa gtcttttaat
     1561 atgcaaagga agttagtaaa gcacagaatt cggcacacgg gggagcggcc ttacagctgc
     1621 tctgcctgcg ggaaatgttt tgggggatca ggtgacctcc gcaggcatgt ccgcactcac
     1681 actggggaga agccgtacac atgtgagatc tgtaacaagt gctttacccg ctctgcggtg
     1741 ctccggcggc acaagaagat gcactgcaaa gctggtgacg agagcccaga tgtgctggag
     1801 gagctcagcc aagccatcga gacctccgac ctcgagaaat ctcagagctc agactctttc
     1861 tcccaagaca cgtctgtgac gctgatgcca gtgtcggtta aactccctgt ccacccagtg
     1921 gaaaattctg tggcagaatt tgatagccac tctggcggct cctattgtaa gttacggtcc
     1981 atgatccaac ctcatggagt tagtgaccag gagaagctga gtttggatcc tggtaaactt
     2041 gccaagcccc agatgcagca gacacagcct caggcctatg cttactcgga tgtggacacc
     2101 ccagccggtg gcgaaccact gcaggccgat ggcatggcca tgatccgttc ctctctggct
     2161 gctttggaca accacggcgg tgaccccctg ggcagtcgag catcttccac cacttatagg
     2221 aactcagagg gtcagttttt ctccagcatg actctctggg ggctagcgat gaagacgctg
     2281 cagaatgaaa acgagttaga ccagtagtaa tgaactagtc cgtaacttga aagtatttcg
     2341 atttcttggc tttatatatc ttgtggaaag gacgaagcaa gtcccgggaa ctgcaaagc
//
