LOCUS       sequence-281531-        3353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1547
FEATURES             Location/Qualifiers
     source          1..3353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1378..1389)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgtcggacag cgaggacagc aacttttccg aggaggagga cagcgagcgc
       61 agcagtgacg gcgaggaggc cgaggtagac gaagagcggc ggagtgcagc gggcagtgag
      121 aaagaagaag agcctgagga cgaagaggag gaggaagagg aggaggaata cgatgaggaa
      181 gaggaggaag aagatgatga ccgacccccc aagaaacccc gccatggagg cttcattctg
      241 gacgaggctg atgttgacga tgagtatgag gacgaggacc agtgggagga tggagcagag
      301 gacattctag agaaagcctc caatatcgat aatgttgtcc tggatgaaga tcgttctggg
      361 gctcgccgcc tgcaaaacct ctggagggac cagcgagaag aagaactggg cgagtattac
      421 atgaagaaat acgccaagtc atctgtggga gagacggtgt atggaggatc tgatgagctc
      481 tcagacgaca tcacccagca gcagctgctc ccaggagtca aggatcccaa tctgtggact
      541 gtcaaatgta agattgggga ggaacgggcc acggccattt ccttgatgcg caagttcatt
      601 gcctaccagt tcacagacac gcccctgcag atcaagtcag tagtggcacc agagcatgtg
      661 aagggctaca tctacgtgga ggcctacaag cagacccacg tgaagcaggc cattgagggg
      721 gtgggcaacc tgcggcttgg ctactggaac cagcagatgg tgcccatcaa ggagatgaca
      781 gacgtgctca aagtggtgaa ggaggtggcc aacctgaaac caaagtcctg ggtccgcctc
      841 aagcggggca tctacaagga tgacattgct caggtggact acgtggagcc cagccagaac
      901 accatctccc tgaagatgat cccacgcatc gactacgatc gcatcaaggc ccgcatgagc
      961 ttgaaagact ggtttgccaa aaggaagaag tttaagcggc ctccacagag gctgtttgat
     1021 gctgagaaga tcaggtccct ggggggtgat gttgcctctg atggtgactt cctcatcttt
     1081 gaggggaacc gttacagccg gaagggcttt ctgttcaaga gcttcgccat gtctgctgtg
     1141 atcacggagg gtgtgaagcc aacactctct gagctggaaa agtttgagga ccagccagag
     1201 ggcattgacc tggaggtggt gactgagagc acagggaagg agcgggagca caacttccaa
     1261 cctggggaca acgtggaggt ctgtgagggt gagctcatca acctgcaggg caagatcctc
     1321 agcgtggatg gcaacaagat caccatcatg cccaagcatg aggacctcaa ggacatgttg
     1381 gagttcccag cccaggaact tagaaaatac ttcaagatgg gggaccacgt gaaggtgatt
     1441 gctggccgat tcgagggcga cacaggcctc attgtgcggg tggaggagaa tttcgttatc
     1501 ctgttctctg acctcaccat gcatgagctg aaggtgctcc cccgggacct gcagctctgc
     1561 tcagagacag catcaggtgt ggatgttggg ggccagcatg aatggggcga gctggtgcag
     1621 ctggatcccc agactgtggg tgtcatcgtg cgactagaac gggagacctt ccaggtgctg
     1681 aacatgtacg ggaaggtggt gactgtcaga catcaggctg tgacccggaa gaaggacaac
     1741 cgctttgctg tggccttgga ctcagagcag aacaacatcc atgtgaaaga catcgttaag
     1801 gtcattgatg gcccccactc aggccgagaa ggggagattc gccatctctt ccgaagcttc
     1861 gccttcctac attgcaagaa actggtggag aacgggggca tgtttgtctg caagacccgc
     1921 cacctggtgc tggctggggg ctcaaagccc cgtgatgtga ccaacttcac cgtgggtggc
     1981 tttgcgccta tgagtccccg gatcagcagc cccatgcacc ccagtgctgg aggtcagcgt
     2041 ggcggctttg gtagcccagg tggcggcagt ggtggcatga gcaggggccg gggccggagg
     2101 gacaacgaac tcatcggcca gaccgtgcgc atctcccagg ggccctacaa aggctacatc
     2161 ggtgtggtga aagatgccac agagtccacg gcccgtgtgg agctgcactc cacctgccag
     2221 accatctctg tggaccgtca gcggctcacc acggtgggct cacggcgccc gggcggcatg
     2281 acctcgacct atgggaggac gcccatgtat ggctcccaga cgcccatgta tggctctggc
     2341 tcccgaacac ccatgtacgg ctcacagaca cccctccagg atggtagccg caccccacac
     2401 tacggctcac agacgcccct gcatgatggc agccgcactc ctgcccagag tggggcctgg
     2461 gaccccaaca accccaacac gccgtcacgg gctgaggaag aatatgagta tgctttcgat
     2521 gatgagccca ccccgtcccc gcaggcctat gggggaaccc ccaatcccca aacacctggc
     2581 tacccagacc cctcgtcccc acaggtcaac ccacaataca acccgcagac gccagggacg
     2641 ccggccatgt acaacacaga ccagttctct ccctatgctg ccccctcccc acaaggttcc
     2701 taccagccca gccccagccc ccagagctac caccaggtgg cgccaagccc agcaggctac
     2761 cagaataccc actccccagc cagctaccac cctacaccgt cgcccatggc ctatcaggct
     2821 agccccagcc cgagccccgt tggctacagt cctatgacac ctggagctcc ctcccctggt
     2881 ggctacaacc cacacacgcc aggctcaggc atcgagcaga actccagcga ctgggtaacc
     2941 actgacattc aggtgaaggt gcgggacacc tacctggata cacaggtggt gggacagaca
     3001 ggtgtcatcc gcagtgtcac ggggggcatg tgctctgtgt acctgaagga cagtgagaag
     3061 gttgtcagca tttccagtga gcacctggag cctatcaccc ccaccaagaa caacaaggtg
     3121 aaagtgatcc tgggcgagga tcgggaagcc acgggcgtcc tactgagcat tgatggtgag
     3181 gatggcattg tccgtatgga ccttgatgag cagctcaaga tcctcaacct ccgcttcctg
     3241 gggaagctcc tggaagccta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3301 tggctttata tatcttgtgg aaaggacgaa cctgcgagat tcttgaacga gaa
//
