LOCUS       sequence-281540-        2375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1578
FEATURES             Location/Qualifiers
     source          1..2375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcccc tgacctggcc cacggaggtc atgtttctag ggactcagtc
       61 tgccttcatg aagaacagac acaggcagca gggatggtgg ctggctggct gataaattgt
      121 taccaggacg cggtgacctt tgacgacgtg gctgtggact tcacccagga ggaatggact
      181 ttactggacc catctcagag agacctctac agagatgtga tgctggaaaa ctacgagaac
      241 ctggcctcag taggacatca cctgttccaa cccagtgtga tctattggct ggagcaggaa
      301 gaggagttga gggcagggcg gagagcagtt ctccaagaat ggcgacttaa aaccaaaggg
      361 ccagcacttc ggcaggatag atcttggttc agagcatcaa atgagacaca gacggcaaga
      421 agccacaatg gagggcagct ctgtgaccgc acgcagtgtg gagaagcttt cagtgaacac
      481 tcaggcctca gcacacacgt gagaactcaa aatacaggag acagttgtgt gtctaatcat
      541 tatgaaaggg acttttttat tccatgccag aaaaccttgt tcaaaattgg agagcagttt
      601 tccgtgttgg gtcagtgtgg aaaagccttc agctctactc caaatgttgt ttcccagcaa
      661 gcatgcactc gggacagatc tcttgactac agcagctgtg gggaagtgtt ccttaatcag
      721 tcataccttc aggcacgtgc gggaagtcac aacggagaag aaacatggaa atggaagccg
      781 tgtgggaaag ctctaactca ctccatgggc tgcgccacac ctgttgaaat gcatgccgtc
      841 aggaatcccc acgtatgtag ggaatgtggg aaggccttta ggtacactgc ctaccttact
      901 ggtcgcgtgc aagtccaccc tggggaaaag ccctgtgaat tggaagaatg tggaaaagcc
      961 tcccctgttt cttccagcct aactcaacat gtaagaattc atgctgcaga gaaaccctgt
     1021 gaatgtaaag aatgcggaaa agccttcact ggactctcag gtctttctaa acacgtccaa
     1081 acagaccctg gacagaagcc ctatgaatgt aaggactgtg ggaaagcctg cggtgggttt
     1141 tatctactga atgagcatgg aaaaactcac acgagggaga agccctttgc atgtgtggtt
     1201 tgcggaaaat attttagaaa ttcctcatgc cttaataatc atgttcgaat tcacactgga
     1261 ataaaaccct atacatgcag ctactgtggg aaggccttca ctgtgcgctg tggccttact
     1321 agacacgtac gaacacacac gggcgagaag ccatacacgt gtaaggactg cgggaaagcc
     1381 ttctgtacat cctcgggcct tactgagcat gtaaggactc acactggaga gaaaccatat
     1441 gaatgtaaag attgtgggaa atccttcact gtttcttcaa gcctgactga gcacgcgaga
     1501 atccataccg gagagaaacc ctacgaatgt aagcagtgtg gcaaagcctt cacagggcgc
     1561 tcaggcctca ctaaacacat gcggacacac accggggaga agccctatga atgtaaggac
     1621 tgtgggaaag cctacaatag ggtttatcta ctgaatgagc atgtgaaaac tcacacagag
     1681 gagaagccct ttatatgtac ggtatgcagg aaatccttca gaaattcctc gtgcctgaat
     1741 aagcacattc agattcacac tggaataaaa ccttatgaat gtaaggactg tgggaaaaca
     1801 ttcactgttt cttcgagcct aaccgagcac atacgaactc acactggaga gaaaccttat
     1861 gaatgtaaag tatgcggaaa ggccttcacc acatcctcac accttatcgt gcacataaga
     1921 acccacaccg gtgagaaacc ctacatatgt aaggagtgtg ggaaagcctt tgcttcctcc
     1981 tcacacctta tcgaacacag aaggactcac acaggagaga aaccttacat atgtaacgag
     2041 tgtgggaaag ccttccgtgc ctcctctcac ctgcataaac atggaagaat tcacactggg
     2101 cagaaaccct ataaatgtaa ggaatgtggg aaagcataca ataggtttta tctactaaaa
     2161 gaacatttaa aaacttacac tgaagagcag gtttttgtat gtaaggactg tggaaaatct
     2221 tttaagaatt cctcatgcct taaccatcac actcaaattc acactgatga gaaacctttc
     2281 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2341 ggaaaggacg actggtcgct aaagcgccaa gccgt
//
