LOCUS       sequence-281552-        1787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1597
FEATURES             Location/Qualifiers
     source          1..1787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctggtgc tcagactctg ttgacgttca gggatgtggc catagaattc
       61 tccctggagg agtggaaatg cctggacctc gctcagcaga atttgtacag ggatgtgatg
      121 ttggagaact acagaaactt gttctccgtt ggtctcactg tctgtaagcc aggcctgatc
      181 acctgcctgg agcaacgaaa agagccctgg aatgtgaaga gacaggaggc agcagacgga
      241 catccagcta tgtcttctca ttttacccaa gaccttctgc cagagcaggg catacaagat
      301 gcattcccaa aaagaatact gagaggatat ggaaattgtg gccttgataa tttatattta
      361 aggaaagact gggaaagttt agatgagtgt aagttgcaaa aagattataa tggacttaac
      421 caatgttcat caactaccca tagcaaaatc tttcaatata ataaatatgt taaaatcttt
      481 gataactttt caaatttaca tagacgtaat ataagtaata ctggagagaa acctttcaaa
      541 tgtcaagaat gtggcaaatc ctttcaaatg ctctcattcc taactgaaca tcagaaaatt
      601 cacactggaa aaaaattcca aaaatgtgga gaatgtggca aaacctttat ccagtgctca
      661 cactttactg aacctgagaa cattgacact ggagagaaac cttacaagtg tcaagaatgt
      721 aacaacgtca ttaaaacttg ctcagtcctt actaaaaata gaatttatgc cggaggggaa
      781 cattacagat gtgaagaatt tggcaaagta tttaaccagt gctcccacct tactgaacat
      841 gagcatggta ctgaggaaaa accctgcaaa tatgaagagt gcagcagtgt ctttatctct
      901 tgctcaagcc tttctaatca acagatgatt cttgctggag agaagctctc caaatgtgaa
      961 acatggtaca aaggttttaa ccacagccca aatccttcca aacaccagag aaatgagatt
     1021 ggagggaaac ctttcaaatg tgaggaatgt gacagcatct tcaagtggtt ctcagacctt
     1081 actaaacata agagaattca cactggtgag aaaccataca aatgtgacga atgtgggaaa
     1141 gcctatacac agtcctcaca cctcagtgaa cacaggagga ttcacaccgg agagaaaccc
     1201 taccaatgtg aagaatgtgg gaaggtcttc agaacttgct caagcctttc taaccataag
     1261 agaactcatt ctgaagaaaa accctacacg tgtgaagaat gtggcaacat ctttaagcag
     1321 ttatcagacc tcactaagca taagaaaacc catactggag agaagcccta caaatgtgac
     1381 gaatgtggaa aaaactttac ccagtcctcc aaccttattg tacataagag aattcatact
     1441 ggagagaaac cctacaagtg tgaagaatgt ggcagagtct tcatgtggtt ctcagacatt
     1501 accaaacata agaaaaccca tactggagag aaaccctaca aatgtgacga atgtggaaaa
     1561 aactttaccc agtcctcaaa ccttattgta cataagagaa ttcatactgg agagaaaccc
     1621 tacaagtgtg aaaagtgtgg caaagccttc acccagttct cacacctgac tgtacatgaa
     1681 agcattcata cttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1741 tatatatctt gtggaaagga cgaagctccc gctcgactca gactgta
//
