LOCUS       sequence-281555-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1602
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgagctgacg gccccggccc agggcattgt gacctttgag
       61 gacgtggctg tttacttctc ctggaaggag tggggtcttc ttgatgaggc tcagaaatgc
      121 ctgtaccacg atgtgatgct ggagaacttg acacttacaa cctccctggg tggttctgga
      181 gcaggggatg aggaggcacc ttatcagcag agcacttctc cacagcgggt gtcacaggtt
      241 aggattccta aggcccttcc ttctccccag aagaccaacc cctgtgagat atgtggccca
      301 gtcttgagac agattttgca cttggttgaa caccaaggaa cacaccatgg tcagaaactg
      361 tatacagacg gggcatgtag gaaacaatta caatttactg cataccttca tcagcaccag
      421 aagcagcatg ttggacagaa acacttcaga agcaatgggg gcagagacat gtttttgagc
      481 agctgcacat ttgaagtatc tgggaagccc ttcacttgca aggaggttgg gaaggatttc
      541 ctggtgagat caagatttct tcagcaacag gctgctcaca ccagaaagaa gtcaaacaga
      601 accaagagtg cagtggcctt tcacagtgta aaaaatcatt acaactgggg agaatgtgtg
      661 aaagctttca gctacaaaca tgtacgtgtt cagcaccagg gagacctcat tagggaaaga
      721 tcttacatgt gcagtgaatg tgggaaatct tttagcacaa gctgtagcct cagtgatcat
      781 ttgagagttc acacttcaga aaagccttat acatgtggag aatgtgggaa atcctatagg
      841 caaagctcta gccttattac gcaccgaaga attcacactg gagtaagacc tcatcaatgt
      901 gatgaatgtg gaaaattatt taacaggaag tatgaccttc ttatacatca gagagttcat
      961 actggagaaa ggccttacaa gtgcagtgaa tgtgggaaat cctttagcca tagctctagc
     1021 ctcattacac accagagaat tcatactgga atgaggcctt atgagtgcag tgaatgtggg
     1081 aaatctttta tccatagttc tagccttatt acacaccaga gagttcacac tggtacaagg
     1141 ccttatatgt gcagtgaatg tgggaaatcc tttagccaga gctgtcacct cattaaacac
     1201 cggagacttc acattggaga agggccttat gagtgtagtg aatgtgggaa attgtttact
     1261 tatagatctc gtttcttcca acaccagaga gttcatactg gagtaagatc tcatgaatgt
     1321 catgaatgtg gaaaattatt tagcaggaaa tttgacctca ttgtacatga gagagttcac
     1381 acaggagaaa ggccatatga gtgcagtgaa tgtggaaaat cctttacctg taaatcctac
     1441 ctcatctcac actggaaagt tcatactgga gcaaggcctt atgaatgtgg ggagtgtggg
     1501 aaatcattta ctcatagctc tacgctcctt caacaccaga gagttcacac tggagaaagg
     1561 ccttatgagt gcaatgaatg tgggaagttt tttagccaga gctccagcct cattagacat
     1621 aggagaagtc acaccggaga aaggccttat gagtgcagtg agtgttggaa atcctttagt
     1681 aaccactcta gcctcgttaa acaccgaaga gttcataccg gagaaaggcc ttatgaatgc
     1741 agtgaatgtg gaaaatcctt tagccagagc tctaacctca ctaatcacca gcgaattcac
     1801 agtggggaaa ggccttatga gtgtagtgac tgtggaaaat tttttacctt caactccaac
     1861 ctcctaaaac atcagaacgt tcacaaggga tagtaatgaa ctagtccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg agtctcagtc cagtaggatg
     1981 tccta
//
