LOCUS       sequence-281561-        2813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1610
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcttc ccgatctact cgtgttacaa gatcaacagt ggggttaaac
       61 ggcttggatg aatctttttg tggtagaact ttaaggaatc gtagcattgc gcatcctgaa
      121 gaaatctctt ctaattctca agtacgatca agatcaccaa agaagagacc agagcctgtg
      181 ccaattcaga aaggaaataa taatgggaga accactgatt taaaacagca gagtacccga
      241 gaatcatggg taagccctag gaaaagagga ctttcttctt cagaaaagga taacatagaa
      301 aggcaggcta tagaaaattg tgagagaagg caaacagaac ctgtttcacc agttttaaaa
      361 agaattaagc gttgtcttag atctgaagca ccaaacagtt cagaagaaga ttctcctata
      421 aaatcagaca aggagtcagt agaacagagg agtacagtag tggacaatga tgcagatttt
      481 caagggacta aacgagcttg tcgatgtctt atactggatg attgtgagaa aagggaaatt
      541 aaaaaggtga atgtcagtga ggaagggcca cttaattctg cagtagttga agaaatcaca
      601 ggctatttgg ctgtcaatgg tgttgatgac agtgattcag ctgttataaa ctgtgatgac
      661 tgtcagcctg atgggaacac taaacaaaat agcattggtt cctatgtgtt acaggaaaaa
      721 tcagtagctg aaaatgggga tacggatacc caaacttcaa tgttccttga tagtaggaag
      781 gaggacagtt atatagacca taaggtgcct tgcacagatt cacaagtgca ggtcaagttg
      841 gaggaccaca aaatagtaac tgcctgcttg cctgtggaac atgttaatca gctgactact
      901 gagccagcta cagggccctt ttctgaaact cagtcatctt taagggattc tgaggaggaa
      961 gtagatgtgg tgggagatag cagtgcctca aaagagcagt gtaaagaaaa caccaataac
     1021 gaactggaca caagtcttga gagtatgcca gcctccggag aacctgaacc atctcctgtt
     1081 ctagactgtg tttcagctca aatgatgtct ttatcagaac ctcaagaaca tcgttatact
     1141 ctgagaacct caccacgaag ggcagcccct accagaggta gtcccactaa aaacagttct
     1201 ccttacagag aaaatggaca atttgaggag aataatctta gtcctaatga aacaaatgca
     1261 actgttagtg ataatgtaag tcaatctcct acaaatcctg gtgaaatttc tcaaaatgaa
     1321 aaagggatat gttgtgactc tcaaaataat ggaagtgaag gagtaagtaa accaccctca
     1381 gaggcaagac tcaatattgg acatttgcca tctgccaaag agagtgccag tcagcacatt
     1441 acagaagagg aagatgatga tcctgatgtt tattactttg aatcagatca tgtggcactg
     1501 aaacacaaca aagattatca gagactatta cagacgattg ctgtactcga ggctcagcgt
     1561 tctcaagcag tccaagacct tgaaagttta ggcaggcacc agagagaagc actgaaaaat
     1621 cccattggat ttgtggaaaa actccagaag aaggctgata ttgggcttcc atatccacag
     1681 agagttgttc aattgcctga gatcgtatgg gaccaatata cccatagcct tgggaatttt
     1741 gaaagagaat ttaaaaatcg taaaagacat actagaagag ttaagctagt ttttgataaa
     1801 gtaggtttac ctgctagacc aaaaagtcct ttagatccta agaaggatgg agagtccctt
     1861 tcatattcta tgttgccttt gagtgatggt ccagaaggct caagcagtcg tcctcagatg
     1921 ataagaggac gcttgtgtga tgataccaaa cctgaaacat ttaaccagtt gtggactgtt
     1981 gaagaacaga aaaagctgga acagctactc atcaaatacc ctcctgaaga agtagaatct
     2041 cgacgctggc agaagatagc agatgaattg ggcaacagga cagcaaaaca ggttgccagc
     2101 cgagtacaga agtatttcat aaagctaact aaagctggca ttccagtacc aggcagaaca
     2161 ccaaacttat atatatactc caaaaagtct tcaacaagca gacgacagca ccctcttaat
     2221 aagcatctct ttaagccttc cactttcatg acttcacatg aaccgccagt gtatatggat
     2281 gaagatgatg accgatcttg ttttcatagc cacatgaaca ctgctgttga agatgcatca
     2341 gatgacgaaa gtattcctat catgtatagg aatttacctg aatataaaga actattacag
     2401 tttaaaaagt taaagaagca gaaacttcag caaatgcaag ctgaaagtgg atttgtgcaa
     2461 catgtgggct ttaagtgtga taactgtggc atagaaccca tccagggtgt tcggtggcat
     2521 tgccaggatt gtcctccaga aatgtctttg gatttctgtg attcttgttc agactgtcta
     2581 catgaaacag atattcacaa ggaagatcac caattagaac ctatttatag gtcagagaca
     2641 ttcttagaca gagactactg tgtgtctcag ggcaccagtt acaattacct tgacccaaac
     2701 tactttccag caaacagata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2761 tggctttata tatcttgtgg aaaggacgac aagcttgtta cgagatgtta cct
//
