LOCUS       sequence-281567-        1580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1625
FEATURES             Location/Qualifiers
     source          1..1580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgatggc actcccgatg cctggacctc aggaggcggt tgtgttcgag
       61 gatgtggctg tgtacttcac aaggatagag tggagttgcc tggcccccga ccagcaggca
      121 ctctacaggg acgtgatgct ggagaactat gggaacctgg cctcactagg ctttcttgtt
      181 gccaaaccag cactgatctc cctattggag caaggagagg agccgggggc cttgattctg
      241 caggtggctg aacagagcgt ggccaaagcc agcctgtgca cagattccag gatggaggct
      301 gggatcatgg agtctcctct gcagagaaag ctctccaggc aggcaggact gccgggcacc
      361 gtgtgggggt gcctcccctg ggggcaccct gtgggggggc accctgcacc accccacccg
      421 catggcggtc ctgaggacgg gtcagataaa cccacccacc cccgggctcg ggagcacagc
      481 gcctccccaa gggttctgca ggaagacctg ggccggcctg tggggagctc agccccccgc
      541 tacaggtgcg tgtgcggcaa ggcgttcaga tacaactcgc tgcttctcag gcaccagatc
      601 atccacaccg gcgccaagcc cttccagtgc acagagtgcg ggaaggcctt caagcaaagc
      661 tccatcctgc tgcggcacca gctgatccac actgaggaga agccgttcca gtgcggcgag
      721 tgcgggaagg ccttccggca gagcacgcag ctggctgccc accaccgcgt ccacacccgc
      781 gagcggccct acgcatgcgg cgagtgcggc aaggccttca gccgcagctc ccggctgctg
      841 cagcaccaga agttccacac cggggagaag cccttcgcgt gcacagagtg cggcaaggcg
      901 ttctgccgca ggttcaccct caacgagcac ggccgcatcc acagcgggga gcggccctac
      961 cggtgcctgc ggtgtgggca gcgcttcatc cgagggtcct cgctcctgaa gcaccaccgg
     1021 ctgcacgcgc aggagggtgc ccaggacggc ggcgtggggc agggcgccct gctcggagct
     1081 gcgcagaggc cccaggcggg ggacccgccc cacgagtgcc cggtgtgcgg gaggccgttc
     1141 cgacacaact ccctgctgct gctgcacctg cgcctacaca cgggcgagaa gccgttcgag
     1201 tgcgcggagt gcggcaaggc cttcggtcgc aagtccaacc tcactctgca ccagaagatc
     1261 cacaccaagg agaagccctt cgcgtgcacc gagtgcggca aggcgttccg caggagctac
     1321 acgctgaacg agcactaccg gctccacagc ggcgagaggc cataccggtg ccgcgcctgc
     1381 gggagggcct gcagccggct gtccaccctc atccagcacc agaaggtgca cggccgcgag
     1441 cccggggagg acacagaggg caggcgggcg ccctgttggg cttcctagta atgaactagt
     1501 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacaat
     1561 cgtacggact ctgtattcag
//
