LOCUS       sequence-281570-        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1627
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtttgta tggacaggct tgtccatctg taacttcatt aaggatgaca
       61 tctgaactgg agagcagcct aacgtctatg gactggttac cacagctcac catgagagca
      121 gccatccaaa aatctgatgc tacacaaaat gcacatggaa caggaatttc taagaagaat
      181 gcactccttg acccaaatac aactctggac caggaagaag tccaacagca caaagatggg
      241 aaacctccat acagttatgc cagcctcatt acatttgcaa ttaatagctc acccaaaaag
      301 aaaatgactt taagtgaaat ttatcagtgg atttgtgata acttcccata ttatagagag
      361 gctggcagtg gttggaagaa ttccatacga cataatctgt cattgaacaa atgtttcctt
      421 aaagtgcctc gatctaagga tgaccctgga aaggggtcct actgggcaat agacaccaat
      481 ccgaaggaag atgtgctgcc tactcggcca aagaagaggg cacgatctgt agaacgggcc
      541 tcaactccat atagcataga ttcagattct ttgggaatgg agtgtattat ttcgggaagt
      601 gcctctccaa ctctggcaat caacactgtg actaacaaag taacattgta taacactgat
      661 caggatggta gtgatagccc acgcagtagc cttaacaaca gtctctcaga ccagagtttg
      721 gcatctgtta atttgaacag tgttggaagt gtgcatagtt atacaccggt gacaagccat
      781 ccagaatcag tctctcaatc attaactcct cagcagcaac cacagtacaa ccttccagaa
      841 agagacaagc aactactttt ctcagaatat aattttgaag atcttagtgc ctcatttcgg
      901 agcctttata agtcagtttt tgagcagtca cttagtcaac aaggtttgat gaacatccct
      961 tctgaatctt cccagcagtc ccacacttca tgtacctatc agcactctcc cagcagtaca
     1021 gtgagcactc acccacacag caaccaaagc agcctgtcca acagtcatgg cagtggcctc
     1081 aacaccacag gcagtaattc ggttgcacag gtctcactgt ctcaccccca gatgcacaca
     1141 cagccatctc cacatcctcc ccatcgaccg catggtttac cgcagcatcc gcagcgttcc
     1201 ccacacccag caccacaccc acagcaacac agccagctcc agtcccctca cccccagcat
     1261 ccctctccac atcaacacat acagcaccat ccgaaccatc agcatcagac gttaacacat
     1321 caggcacccc cacccccaca acaggtatcc tgtaattctg gtgtttcaaa tgattggtat
     1381 gcgacacttg atatgctaaa agaaagctgt cgaattgcca gcagtgttaa ttggtcagat
     1441 gtagaccttt cacagtttca aggtctgatg gagagtatga gacaggcaga tctcaagaac
     1501 tggtctttag accaggttca gtttgccgat ctttgttctt ctcttaatca gttctttaca
     1561 caaactggcc ttatacattc acagagtaat gttcaacaaa atgtttgtca tggtgccatg
     1621 catccaacaa aaccttccca acacattgga acaggaaatt tgtacataga ttctaggcaa
     1681 aatctccctc cttcagtgat gccaccccct ggttatcctc atatcccaca ggcactcagc
     1741 actccaggaa caacgatggc aggccatcac agagccatga accagcagca catgatgcct
     1801 tcccaagcct tccagatgcg gcgttccctg cctccagatg acatccagga tgactttgat
     1861 tgggattcaa ttgtgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1921 ctttatatat cttgtggaaa ggacgatgga gaacagccct gggtggcata
//
