LOCUS       sequence-281576-        1748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1685
FEATURES             Location/Qualifiers
     source          1..1748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgtccaa gctcacgtcg ctccagcaag aactcctgag cgccctgctg
       61 agctccgggg tcaccaagga ggtgctggtt caggccttgg aggagttgct gccatccccg
      121 aacttcgggg tgaagctgga gacgctgccc ctgtcccctg gcagcggggc cgagcccgac
      181 accaagccgg tcttccatac tctcaccaac ggccacgcca agggccgctt gtccggcgac
      241 gagggctccg aggacggcga cgactatgac acacctccca tcctcaagga gctgcaggcg
      301 ctcaacaccg aggaggcggc ggagcagcgg gcggaggtgg accggatgct cagtgaggac
      361 ccttggaggg ctgctaaaat gatcaagggt tacatgcagc aacacaacat cccccagagg
      421 gaggtggtcg atgtcaccgg cctgaaccag tcgcacctct cccagcatct caacaagggc
      481 acccctatga agacccagaa gcgtgccgct ctgtacacct ggtacgtcag aaagcaacga
      541 gagatcctcc gacaattcaa ccagacagtc cagagttctg gaaatatgac agacaaaagc
      601 agtcaggatc agctgctgtt tctctttcca gagttcagtc aacagagcca tgggcctggg
      661 cagtccgatg atgcctgctc tgagcccacc aacaagaaga tgcgccgcaa ccggttcaaa
      721 tgggggcccg cgtcccagca aatcttgtac caggcctacg atcggcaaaa gaaccccagc
      781 aaggaagaga gagaggcctt agtggaggaa tgcaacaggg cagaatgttt gcagcgaggg
      841 gtgtccccct ccaaagccca cggcctgggc tccaacttgg tcactgaggt ccgtgtctac
      901 aactggtttg caaaccgcag gaaggaggag gcattccggc aaaagctggc catggacgcc
      961 tatagctcca accagactca cagcctgaac cctctgctct cccacggctc cccccaccac
     1021 cagcccagct cctctcctcc aaacaagctg tcaggagtgc gctacagcca gcagggaaac
     1081 aatgagatca cttcctcctc aacaatcagt caccatggca acagcgccat ggtgaccagc
     1141 cagtcggttt tacagcaagt ctccccagcc agcctggacc caggccacaa tctcctctca
     1201 cctgatggta aaatgatctc agtctcagga ggaggtttgc ccccagtcag caccttgacg
     1261 aatatccaca gcctctccca ccataatccc cagcaatctc aaaacctcat catgacaccc
     1321 ctctctggag tcatggcaat tgcacaaagc ctcaacacct cccaagcaca gagtgtccct
     1381 gtcatcaaca gtgtggccgg cagcctggca gccctgcagc ccgtccagtt ctcccagcag
     1441 ctgcacagcc ctcaccagca gcccctcatg cagcagagcc caggcagcca catggcccag
     1501 cagcccttca tggcagctgt gactcagctg cagaactcac acatgtcctc tacaagcctg
     1561 gtgatgccca cacaccactt acttcgtgcg caacaacaag gaccctgttt tccacaccat
     1621 caccctctgg gcagctgtca tggaaaagcc cagtagtaat gaactagtcc gtaacttgaa
     1681 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagtactg cacgacacga
     1741 caactgtc
//
